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[1]肖蓉,王媛,聂园军,等.基于高通量测序技术的铬污染农田土壤菌群多样性及修复菌株的筛选[J].应用与环境生物学报,2019,25(04):933-942.[doi:10.19675/j.cnki.1006-687x.2018.08024]
 XIAO Rong,WANG Yuan,NIE Yuanjun**,et al.Research on bacterial communities in Cr-polluted farmland soil by high-throughput sequencing and selection of Cr remediation strains[J].Chinese Journal of Applied & Environmental Biology,2019,25(04):933-942.[doi:10.19675/j.cnki.1006-687x.2018.08024]
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基于高通量测序技术的铬污染农田土壤菌群多样性及修复菌株的筛选
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年04期
页码:
933-942
栏目:
研究论文
出版日期:
2019-08-25

文章信息/Info

Title:
Research on bacterial communities in Cr-polluted farmland soil by high-throughput sequencing and selection of Cr remediation strains
作者:
肖蓉;?王媛;?聂园军;?程滨;?赵佳;?曹秋芬
1山西省农业科学院果树研究所 太原 030031 2山西省农业科学院农业资源与经济研究所 太原 030006 3山西省农业科学院农业环境与资源研究所 太原 030031 4山西省农业科学院生物技术研究中心 太原 030031
Author(s):
XIAO Rong1;? WANG Yuan1;? NIE Yuanjun2**;? CHENG Bin3;? ZHAO Jia4 & CAO Qiufen4
1 Pomology Institute, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China 2 Institute of Agricultural Resources and Economy, Shanxi Academy of Agricultural Sciences, Taiyuan 030006, China 3 Institute of Agricultural Environment and Resource, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China 4 Biotechnology Research Center, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China
关键词:
高通量测序;?细菌多样性;?耐铬;?纤维微菌属;?快速筛选
Keywords:
high-throughput sequencing;? bacterial community diversity;? chromium resistance;? Cellulosimicrobium;? rapid screening
分类号:
S154.36 : X172
DOI:
10.19675/j.cnki.1006-687x.2018.08024
摘要:
菌种资源筛选是铬污染土壤生物修复的基础. 以铬污染农田土壤为研究对象,采用高通量测序方法分析土壤细菌多样性特征,并根据多样性分析结果,采用选择性培养基和培养方法快速筛选对铬具有适应性和去除能力的细菌,以寻找能在铬污染农田土壤原位修复中具有较大应用潜力的菌种资源. 结果显示:受铬污染的土壤细菌群落丰富度和多样性均低于未受铬污染土壤. 门水平上,铬污染土壤中放线菌门(Actinobacteria)细菌丰度显着高于未受铬污染的土壤,是两种生境丰度差异最大的门. 属水平上,芽孢杆菌属(Bacillus)是铬污染土壤中丰度最高的优势属,显着高于未受铬污染的土壤. 从铬污染土壤中共分离到6株能够耐受1 000 μg/mL铬的细菌. 6株菌都具有一定的六价铬Cr(VI)去除能力,其中Cr1、Cr3和Cr8能在72 h内将500 μg/mL铬培养基中的Cr(VI)全部去除,Cr8能在72 h内将1 000 μg/mL铬培养基中的Cr(VI)去除61.2%. 16S rDNA测序结果表明,6株菌中有5株属于厚壁菌门芽孢杆菌属,1株属于放线菌门纤维微菌属(Cellulosimicrobium). 结合菌体及菌落形态特征和序列分析结果,铬去除能力最高的Cr8菌可初步被确定为C. aquatile. 本研究表明铬污染降低土壤中细菌多样性,根据高通量测序结果有选择性地快速筛选铬污染修复菌株具有可行性,Cr8菌是国内首次分离到的具有铬污染修复功能的纤维微菌属菌株. (图11 表5 参40)
Abstract:
The basis for bioremediation of chromium-contaminated soil is screening bacterial resources. In this study, soil bacterial communities from a Cr-contaminated farmland were analyzed by high-throughput sequencing. Based on the sequencing results, a series of enrichment methods was applied to isolate Cr-resistant bacteria to find candidate strains for in situ bioremediation of Cr-contaminated farmland soil. The community richness and diversity indexes were significantly lower in Cr-contaminated soil than in uncontaminated soil. A community composition analysis showed that contaminated soil had a higher richness of Actinobacteria at the phylum level as well as enriched species from Bacillus at the genus level. Six bacterial strains, isolated from Cr-contaminated soil, were able to grow in a culture medium containing 1 000 μg/mL Cr. These strains could reduce the Cr(VI) content to some extent, and three strains—Cr1, Cr3, and Cr8—removed all the Cr(VI) in an LB medium containing 500 μg/mL Cr within 72?hours. The strain Cr8 removed?61.2% of the Cr(VI) when cultured in an LB medium containing 1 000 μg/mL Cr within 72?hours. Molecular identification of the strains revealed that five isolates were from the genus Bacillus (Firmicutes) and one was from the genus Cellulosimicrobium (Actinobacteria). Finally, strain Cr8 was identified as Cellulosimicrobium aquatile based on cell and colony morphology and molecular identification. Our work shows that Cr contamination leads to reduced soil bacterial diversity. High-throughput sequencing is a powerful tool to further screen for bacterial strains with Cr remediation potential. To our knowledge, this is the first report in China to show that strains of Cellulosimicrobium are effective in remediating Cr-contaminated soil.

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更新日期/Last Update: 2019-08-25