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[1]岳士忠,张慧琦,黄财德,等.不同基质及硒添加量对蚯蚓生长、繁殖和富硒能力的影响[J].应用与环境生物学报,2019,25(04):885-891.[doi:10.19675/j.cnki.1006-687x.2018.10014]
 YUE Shizhong,ZHANG Huiqi,HUANG Caide,et al.Effects of different culture materials and selenium contents on the growth, reproduction, and tissue selenium enrichment of the earthworm Eisenia fetida[J].Chinese Journal of Applied & Environmental Biology,2019,25(04):885-891.[doi:10.19675/j.cnki.1006-687x.2018.10014]
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不同基质及硒添加量对蚯蚓生长、繁殖和富硒能力的影响
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年04期
页码:
885-891
栏目:
研究论文
出版日期:
2019-08-25

文章信息/Info

Title:
Effects of different culture materials and selenium contents on the growth, reproduction, and tissue selenium enrichment of the earthworm Eisenia fetida
作者:
岳士忠;?张慧琦;?黄财德;?乔玉辉
中国农业大学资源与环境学院 北京 100193
Author(s):
YUE Shizhong;? ZHANG Huiqi;? HUANG Caide;? QIAO Yuhui**
College of Resources and Environment, China Agricultural University, Beijing 100193, China
关键词:
赤子爱胜蚓;?农业废弃物;?生物量;?繁殖;?硒含量;?富集系数
Keywords:
Eisenia fetida;? agricultural waste;? biomass;? reproduction;? selenium concentration;? bioaccumulation factor
分类号:
S185
DOI:
10.19675/j.cnki.1006-687x.2018.10014
摘要:
蚯蚓是潜在的富硒蛋白饲料. 在大棚环境条件下采用不同配比的3种农业废弃物(牛粪、菌渣和残余菜叶)和低浓度硒(Na2SeO3,0/20/40 mg/kg)对赤子爱胜蚓(Eisenia fetida)进行培养,研究不同培养基质和硒添加量对蚯蚓生长繁殖和富硒能力的影响. 结果发现,培养45 d后,与相同浓度硒处理的其他3组基质[纯牛粪、牛粪+菌渣+菜叶(干重比为6/2/2和3/6/1)]相比,牛粪+菌渣组(干重比为1/1)蚯蚓总生物量、蚓茧数、蚓体硒富集量和富集系数均达到最大,其中总生物量比培养前最大增加了18.21%,每100条蚯蚓产蚓茧数量达68.35-72.45个,蚓体硒富集量最高达到151.56 mg/kg,蚓体硒富集系数最大为4.91. 当硒添加浓度为20 mg/kg和40 mg/kg时,以牛粪+菌渣+菜叶为基质的两组(干重比为6/2/2,3/6/1)在培养初期对蚯蚓活性和培养后的总生物量增加均有一定的抑制作用,蚯蚓总生物量最大降低了8.48%. 在不同基质培养条件下,外源添加硒对蚓体硒富集量和硒富集系数增加有促进作用,蚓体硒富集量与基质硒添加量均呈现出剂量-效应关系. 上述结果表明,赤子爱胜蚓对硒具有极强的富集能力且培养基质种类对蚯蚓的生长繁殖具有很大的影响,牛粪和菌渣组合(干重比为1/1)是最佳的富硒蚯蚓培养基质且基质硒添加浓度≤ 40 mg/kg不影响蚯蚓生长繁殖. (图3 表4 参25)
Abstract:
This study investigates the effects of three different agricultural wastes and selenite doses on earthworm growth, reproduction, and selenium (Se) enrichment in its tissues. Earthworms were cultured with three agricultural wastes (fermented cow manure, mushroom residue, and vegetable residue) and low concentrations of selenite (0/20/40 mg/kg) for 45 days under greenhouse conditions. After 45 days of cultivation, the total biomass gain, cocoon production, Se bioaccumulation, and Se bioaccumulation factors (BAFSe) in earthworm tissues were the highest when exposed to cow manure and mushroom residue (mass ration: m/m = 1/1) than to the other three different substrates (pure cow manure, cow manure + mushroom residue + vegetable residue, m/m/m = 6/2/2 and 3/6/1). The maximum total biomass increased by 18.21%, compared to their initial weight. The number of cocoons reached 68.35-72.45 per 100 earthworms. Se bioaccumulation concentration in earthworm tissues was 151.56 mg/kg dry weight. The substrate of cow manure and mushroom residue had a maximum value of Se BAFSe, equal to 4.91. When Se addition in substrates, based on cow manure + mushroom residue + vegetable residue (m/m/m = 6/2/2 and 3/6/1), was 20 and 40 mg/kg, the activities of earthworms in the early stage of cultivation were inhibited. The increase of total biomass after cultivation was also inhabited. The total earthworm biomass decreased by 8.48%. Se bioaccumulation amounts in earthworm tissues were significantly affected by the selenite doses in the four different substrates, which shows a dose-dependent relationship. In conclusion, the substrate types have a significant influence on the growth and reproduction of earthworms. E. fetida has strong enrichment ability for Se. The cow manure and the mushroom residue were the best substrates. Se addition with less than 40 mg/kg did not affect the growth and reproduction of earthworms in this study.

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更新日期/Last Update: 2019-08-25