|本期目录/Table of Contents|

[1]路文硕,田兴军,王强.常见离子对漆酶降解活性艳蓝的调控作用[J].应用与环境生物学报,2019,25(04):959-965.[doi:10.19675/j.cnki.1006-687x.2018.10031]
 LU Wenshuo,TIAN Xingjun & WANG Qiang**.Regulation effects of common ions on the degradation rate of reactive brilliant blue decomposed by laccase[J].Chinese Journal of Applied & Environmental Biology,2019,25(04):959-965.[doi:10.19675/j.cnki.1006-687x.2018.10031]
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常见离子对漆酶降解活性艳蓝的调控作用
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年04期
页码:
959-965
栏目:
研究论文
出版日期:
2019-08-25

文章信息/Info

Title:
Regulation effects of common ions on the degradation rate of reactive brilliant blue decomposed by laccase
作者:
路文硕;?田兴军;?王强
南京大学生命科学学院 南京 210023
Author(s):
LU Wenshuo;? TIAN Xingjun & WANG Qiang**
School of Life Sciences, Nanjing University, Nanjing 210023, China
关键词:
漆酶;?漆酶–介体系统;?活性艳蓝;?离子;?降解率;?活性位点
Keywords:
laccase;? laccase-mediator system;? reactive brilliant blue;? ion;? degradation rate;? active site
分类号:
Q554 : X703
DOI:
10.19675/j.cnki.1006-687x.2018.10031
摘要:
漆酶具有很高的工业价值,但水体中离子的存在为其推广使用带来了巨大挑战. 探讨水体中常见的7种阳离子和8种阴离子调控自由漆酶和漆酶–介体系统对水中活性艳蓝降解速率的差异. 结果显示:Mn2+、Cu2+、Zn2+、HPO42- 和NO2-在0-10 mmol/L范围内可促进漆酶对活性艳蓝的降解,降解速率由高到低依次为NO2-、Mn2+、Cu2+、Zn2+、HPO42-;SO32- 、Cl-、Fe2+和Fe3+对活性艳蓝的降解有抑制作用,其中Fe2+和SO32-的最低抑制浓度分别为5和0.5 mmol/L;其他离子对漆酶降解活性艳蓝没有显着性影响. 在5种介体的协同作用中,紫脲酸(VA)的效果最佳,导致漆酶对活性艳蓝的降解速率提升10%左右,其余介体的促进效率明显低于VA. 在同等离子的调控下,漆酶–介体系统中的降解速率优于自由漆酶. 本研究初步揭示了漆酶对活性艳蓝的降解机理,同时可以通过控制水体中离子的种类和数量来更好地实现漆酶对活性艳蓝的降解,结果可为漆酶在实际染料废水处理中的应用提供理论依据. (图4 表4 参40)
Abstract:
Laccase plays a key role in degrading dye wastewater; however, a major challenge is the presence of various ions that limit the potential application of the enzyme in bioremediation. This study compared the degradation rates of reactive brilliant blue regulated by seven metal ions and eight anions in the free laccase system and laccase-mediator system. The results showed that Mn2+, Cu2+, Zn2+, HPO42-, and NO2- can promote the degradation rates of reactive brilliant blue decomposed by laccase. The degradation rates from highest to lowest were NO2-, Mn2+, Cu2+, Zn2+, and HPO42-. In contrast, the addition of Cl-, Fe2+, or Fe3+ at low doses significantly inhibited degradation rate of reactive brilliant blue decomposed by laccase. The minimum inhibitory concentrations of Fe2+ and SO32- were 5 mmol/L and 0.5 mmol/L, respectively. Moreover, the degradation rates were not significantly different when mediated by other ions. We also selected five redox-mediating compounds that follow distinct oxidation pathways during RBBR degradation. The laccase-VA system showed the highest degradation rate of reactive brilliant blue, approximately 10% higher than that in the pure laccase system. The degradation rate in the laccase-mediator system was better than that in the free laccase system when under the control of equivalent ions. Therefore, to provide theoretical bases for the application of laccase in the treatment of dye wastewater, this study aimed to analyze the degradation rates of reactive brilliant blue by controlling the types and concentrations of ions in the free laccase and laccase-mediator systems.

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更新日期/Last Update: 2019-08-25