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[1]李娜,彭乾,何健,等.麦草畏降解菌株Sphingobium sp. Dca-5的分离、鉴定及降解特性[J].应用与环境生物学报,2019,25(04):943-949.[doi:10.19675/j.cnki.1006-687x.2018.11002]
 LI Na**,PENG Qian,HE Jian,et al.Isolation, identification, and degradation characteristics of a dicamba-degrading bacterium Sphingobium sp. Dca-5[J].Chinese Journal of Applied & Environmental Biology,2019,25(04):943-949.[doi:10.19675/j.cnki.1006-687x.2018.11002]
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麦草畏降解菌株Sphingobium sp. Dca-5的分离、鉴定及降解特性
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年04期
页码:
943-949
栏目:
研究论文
出版日期:
2019-08-25

文章信息/Info

Title:
Isolation, identification, and degradation characteristics of a dicamba-degrading bacterium Sphingobium sp. Dca-5
作者:
李娜;?彭乾;?何健;?张浩;?黄红慧;?李景照;?惠丰立
1南阳师范学院生命科学与技术学院 南阳 473061 2南京农业大学生命科学学院 南京 210095
Author(s):
LI Na1**;? PENG Qian2;? HE Jian2;? ZHANG Hao1;? HUANG Honghui1;? LI Jingzhao1;? HUI Fengli1
1 School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China 2 College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China
关键词:
麦草畏;?分离鉴定;?Sphingobium sp. Dca-5;?脱甲基酶;?代谢途径;?细胞色素P450类单加氧酶
Keywords:
dicamba;? isolation and identification;? Sphingobium sp. Dca-5;? demethylase;? metabolic pathway;? cytochrome P450 monooxygenase
分类号:
X172
DOI:
10.19675/j.cnki.1006-687x.2018.11002
摘要:
麦草畏是一种广谱高效除草剂,随着抗麦草畏转基因作物的推广和使用,麦草畏的使用量急剧增加. 为研究麦草畏的微生物降解机制,发掘新的麦草畏高效降解菌株和基因资源,从农药厂排污口土壤中筛选到一株以麦草畏为唯一碳源生长的鞘酯菌属(Sphingobium sp.)细菌Dca-5. 该菌株48 h内完全矿化2.0 mmol/L的麦草畏,并检测到代谢产物3,6-二氯水杨酸,表明该菌株通过脱甲基起始麦草畏降解. Dca-5粗酶液在添加NADH时具有麦草畏脱甲基酶活性,但加入细胞色素P450抑制剂1-氨基苯并三唑、增效醚或马拉硫磷后酶活受到显着抑制. 在Dca-5基因组中找到两个3,6-二氯水杨酸降解基因簇但未发现DMO和dmt同源基因. 结果表明菌株Dca-5中的麦草畏脱甲基酶是一个新的细胞色素P450类单加氧酶. 本研究为阐明麦草畏的微生物降解代谢机制,获得新的麦草畏脱甲基酶基因提供了菌株材料和理论依据;并且Sphingobium sp. Dca-5能快速矿化麦草畏,在麦草畏污染土壤的微生物修复方面具有良好的应用前景. (图8 表1 参27)
Abstract:
Dicamba is a broad-spectrum high-efficiency herbicide; the Monsanto Biotechnology Company has successfully constructed dicamba-resistant genetically modified crops, which have been commercially grown on a large scale. The annual use of dicamba has significantly increased over the past years. This study aimed to isolate new dicamba-degrading strains and new dicamba-degrading gene resources. A dicamba-degrading bacterium Dca-5, identified as Sphingobium sp. strain Dca-5, isolated from sludge in the wastewater outlet of a pesticide plant, could completely degrade 2.0 mmol/L dicamba within 48 h. 3,6-Dichlorosalicylate (3,6-DCSA) was identified as an intermediate, indicating that the first step in dicamba degradation is the demethylation of strain Dca-5. The crude enzyme of strain Dca-5 showed dicamba demethylase activity only in the presence of NADH, and the dicamba demethylase activity was severely inhibited by the addition of 1-aminobenzotriazole (ABT), piperonyl butoxide (PBO), or malathion, which are cytochrome P450 inhibitors. Furthermore, the previously reported dicamba demethylase genes DMO and dmt were not present in the genome of strain Dca-5. Based on the above analyses, a dicamba-utilizing bacterium—Sphingobium sp. Dca-5—was isolated; the dicamba demethylase in strain Dca-5 is a novel cytochrome P450-type monooxygenase that differs from previously reported demethylases. This study facilitates further elucidation of dicamba catabolism and provides an excellent strain for cloning a dicamba-detoxification gene. Furthermore, strain Dca-5 could rapidly mineralize dicamba, making it a good candidate for bio-remediation of dicamba-contaminated environments.

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更新日期/Last Update: 2019-08-25