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[1]廖泽东,王雪梅,卢志宇,等.香叶醇联合柠檬醛体外抗MRSA的优化配比[J].应用与环境生物学报,2019,25(04):897-901.[doi:10.19675/j.cnki.1006-687x.2018.11021]
 LIAO Zedong,WANG Xuemei,et al.In vitro antibacterial activity and the optimum ratio of geraniol combined with citral against MRSA[J].Chinese Journal of Applied & Environmental Biology,2019,25(04):897-901.[doi:10.19675/j.cnki.1006-687x.2018.11021]
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香叶醇联合柠檬醛体外抗MRSA的优化配比
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年04期
页码:
897-901
栏目:
研究论文
出版日期:
2019-08-25

文章信息/Info

Title:
In vitro antibacterial activity and the optimum ratio of geraniol combined with citral against MRSA
作者:
廖泽东;?王雪梅;?卢志宇;?周洋莹;?代敏;?孙丰慧
1成都医学院四川省动物源性食品兽药残留防控技术工程实验室 成都 610500 2成都医学院检验医学院 成都 610500
Author(s):
LIAO Zedong1;? 2;? WANG Xuemei1;? 2;? LU Zhiyu1;? 2;? ZHOU Yangying1;? 2;? DAI Min1;? 2 & SUN Fenghui1;? 2**
1 Sichuan Provincial Engineering Laboratory for Prevention and Control Technology of Veterinary Drug Residue in Animal-origin Food, Chengdu Medical College, Chengdu, 610500, China 2 School of Laboratory Medicine, Chengdu Medical College, Chengdu, 610500, China
关键词:
香叶醇;?柠檬醛;?耐甲氧西林金黄色葡萄球菌;?联合用药;?体外抑菌活性
Keywords:
geraniol;? citral;? methicillin-resistant Staphylococcus aureus (MRSA);? drug combination;? in vitro antibacterial activity
分类号:
R962
DOI:
10.19675/j.cnki.1006-687x.2018.11021
摘要:
通过体外抑菌实验研究香叶醇与柠檬醛联合使用抗耐甲氧西林金黄色葡萄球菌(Methicillin resistant Staphylococcus aureus,MRSA)的体外抑菌活性,并采用单纯形格子混料设计优化二者比例. 采用微量稀释法测定香叶醇和柠檬醛对43株MRSA的最低抑菌浓度(Minimum inhibitory concentration,MIC)和最低杀菌浓度(Minimum bactericidal concentration,MBC),采用棋盘法测定香叶醇和柠檬醛联合用药时的分级抑菌浓度(Fractional inhibitory concentration,FIC),采用单纯形格子设计优化香叶醇和柠檬醛联合用药时的比例,并采用微量稀释法对最佳配比混合液的抗MRSA活性进行测定. 结果显示:香叶醇的MIC值为0.34-0.67 mg/mL,柠檬醛的MIC值为0.66-42.23 mg/mL. 二者联合使用时,以协同作用为主,占46.51%;其次为相加作用,占41.86%. 单纯形格子设计优化的香叶醇和柠檬醛联合使用的最佳比例为香叶醇84.50%、柠檬醛15.50%. 采用此配比测定香叶醇和柠檬醛混合液对43株MRSA的MIC值,发现混合液MIC50、MIC90分别为0.25 mg/mL和0.44 mg/mL;与香叶醇单独使用相比,MIC50和MIC90分别约为单独使用时的1/2和 2/3;与柠檬醛单独使用相比,MIC50和MIC90分别约为单独使用时的1/3和1/4. 上述结果表明香叶醇与柠檬醛联合使用时可起到增敏的效果,且降低彼此的用量. (图2 表5 参31)
Abstract:
In vitro antibacterial activity of geraniol combined with citral against methicillin-resistant Staphylococcus aureus (MRSA) was studied. It was attempted to optimize the ratio of geraniol and citral by the simple lattice mixture design test. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of geraniol and citral against 43 strains of MRSA were determined by the microdilution method. Also, the partial inhibitory concentration (FIC) of geraniol combined with citral was determined by the chessboard method. A simplex lattice design test was used to optimize the ratio of geraniol and citral. The MIC values of the optimum mixture against the tested MRSA strains were determined. The results showed that the MIC values of geraniol and citral were 0.34-0.67 mg/mL and 0.66-42.23 mg/mL, respectively. When the geraniol and citral were combined, the synergistic effect was the main factor, accounting for 46.51%, followed by the additive effect, accounting for 41.86%. A simplex lattice design model was established to optimize the ratio of geraniol and citral in combination. The optimum ratio was 84.50% geraniol and 15.50% citral. The MIC values of the optimum mixture against the tested MRSA strains were determined. It was found that the MIC50 and MIC90 of the mixed solution were 0.25 mg/mL and 0.44 mg/mL, respectively. When compared to geraniol alone, the MIC50 and MIC90 were about 1/2 and 2/3 of the geraniol. When compared to citral alone, the MIC50 and MIC90 were about 1/3 and 1/4 of the citral. Therefore, geraniol combined with citral exhibit better antimicrobial activity against MRSA and the dosage of geraniol and citral can be significantly reduced.

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更新日期/Last Update: 2019-08-25