|本期目录/Table of Contents|

[1]张君诚,宋育红,朱勇,等.长柄石杉居群遗传多样性和遗传结构的AFLP分析[J].应用与环境生物学报,2011,17(01):18-23.[doi:10.3724/SP.J.1145.2011.00018]
 ZHANG Juncheng,SONG Yuhong,ZHU Yong,et al.AFLP Analysis of Genetic Diversity and Population Structure of Huperzia serrata (Thunb. ex Murray) Trev. var. longipetiolata (Spring) H. M. Chang[J].Chinese Journal of Applied & Environmental Biology,2011,17(01):18-23.[doi:10.3724/SP.J.1145.2011.00018]
点击复制

长柄石杉居群遗传多样性和遗传结构的AFLP分析()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
17卷
期数:
2011年01期
页码:
18-23
栏目:
研究论文
出版日期:
2011-02-25

文章信息/Info

Title:
AFLP Analysis of Genetic Diversity and Population Structure of Huperzia serrata (Thunb. ex Murray) Trev. var. longipetiolata (Spring) H. M. Chang
作者:
张君诚;?宋育红;?朱勇;?张杭颖;?钟扬
(1三明学院应用生物技术研究所 三明 365004)
(2复旦大学生物多样性与生态工程教育部重点实验室 上海 200433)
Author(s):
ZHANG Juncheng;? SONG Yuhong;? ZHU Yong;? ZHANG Hangying & ZHONG Yang
(1Institute of Applied Biotechnology, Sanming College, Sanming 365004, Fujian, China)
(2Key Laboratory of Biodiversity Science & Ecology Engineer, Ministery of Education, Fudan University, Shanghai 200433, Fujian, China)
关键词:
长柄石杉;?AFLP;?遗传多样性;?居群;?遗传结构;?武夷山
Keywords:
Huperzia serrata (Thunb. ex Murray) Trev. var. longipetiolata (Spring) H. M. Chang;? AFLP;? genetic diversity;? population genetics;? genetic structure;? Wuyi Mountains
分类号:
Q949.360.3
DOI:
10.3724/SP.J.1145.2011.00018
文献标志码:
A
摘要:
石杉科植物因所含石杉碱甲(Huperzine A)对中老年痴呆等具有良好疗效,近年来倍受关注. 利用AFLP分子标记对武夷山脉广布种长柄石杉[Huperzia serrata (Thunb. ex Murray) Trev. var. longipetiolata (Spring) H. M. Chang] 7个居群112株个体进行遗传多样性和居群遗传结构分析. 选用多态性高、分辨力强的8对选择性扩增引物组合共获得675个位点,其中多态位点比例为69.38%. 居群内观测等位基因数(Na)为1.633,有效等位基因数(Ne)1.493;Nei’s基因多样性指数(He)与Shannon多态性信息指数(I)的平均值分别为0.272和0.392,多样性最高为地处武夷山脉中段的泰宁居群和建宁居群,最低为山脉北段的光泽居群. 居群总基因多样性(Ht)为0.327 3,居群内基因多样性(Hs)为0.272 2,居群间的遗传分化系数(Gst)为0.168 1,表明居群内变异是长柄石杉遗传多样性的主要来源. 由Gst估计,武夷山脉长柄石杉自然居群的基因流(Nm)为2.474 0. 邻接树分析表明居群间遗传亲缘关系与地理位置相关. 武夷山脉长柄石杉较高的遗传多样性和基因流水平表明其仍然具有相当的适应(生存)能力和进化潜力,这可能与其生物学(异交水平)、生态学特性及武夷山脉相对良好的生境条件有关. 图2 表5 参41
Abstract:
Huperziaceae has attracted the attention of researchers in recent years because of its Huperzine A (HupA) being a potent, reversible and selective acetylcholinesterase inhibitor (AChEI). The genetic diversity and genetic structure of seven populations and 112 individuals of Huperzia serrata (Thunb. ex Murray) Trev. var. longipetiolata (Spring) H. M. Chang from the Wuyi Mountains were analyzed by amplified fragment length polymorphism (AFLP). We recorded a total of 675 amplified bands using 8 pairs of AFLP primers, 555 of which (PPB = 69.38%) were polymorphic. The observed number of alleles (Na) and effective number of alleles (Ne) were 1.633 and 1.493, respectively. The average values of Nei’s genetic diversity index (He) and Shannon polymorphism information index (I) were 0.272 and 0.392, respectively; the Taining and Jianning populations located in the intermediate section of the Wuyi Mountains showed the highest diversity, while the Guangze population distributed in the north section of the Mountains had the lowest diversity. The total genetic diversity (Ht) was 0.327 3, and the population gene diversity (Hs) was 0.272 2. The coefficient of gene differentiation among populations within species (Gst) was 0.168 1, suggesting that intra-population variation is the main source of the genetic diversity of H. serrata var. longipetiolata from the Wuyi Mountains. Estimated from Gst, the gene flow (Nm) of this natural population was 2.474. Adjacent tree analysis (NJ) showed that the genetic sibship among/between populations was correlated with geographical location. This study provides a profound basis for the resource protection of H. serrata var. longipetiolata. Fig 2, Tab 5, Ref 41

参考文献/References:

1 Wang YE (王月娥), Yue DX (岳东贤), Tang XC (唐希灿). Anti-cholinesterase activity of huperzine A. Acta Pharmacol Sin (中国药理学报), 1986, 7 (2): 110
2 Ma XQ, Gang DR. The Lycopodium alkaloids. Nat Prod Rep, 2004, 21 (6): 752~772
3 Ma XQ, Tan CH, Zhu DY, Gang DR. Is there a better source of huperzine A than Huperzia serrata? Huperzine A content of Huperziaceae species in China. J Agric & Food Chem, 2005, 53: 1393~1398
4 Ma XQ, Tan CH, Zhu D, Xiao P. Huperzine A from Huperzia species- an ethnopharmacological review. J Ethnopharmacol, 2007, 113 (1): 15~34
5 Wang J (王峻), Pan SL (潘胜利). Study on content of huperzine A in six plants of Huperzia from Hunan province. Chin Pharm J (中国药学杂志), 2005, 40 (21): 1616~1618
6 Ji SG (姬生国), Pan SL (潘胜利), Wang J (王峻). Phylogeny relationship and molecular identification of ten Huperzia species (Huperziaceae) based on matK gene sequeuces. China J Chin Mat Med (中国中药杂志), 2007, 32 (19): 1971~1975
7 Ji SG, Huo KK, Wang J, Pan SL. A molecular phylogenetic study of Huperziaceae based on chloroplast rbcL and psbA-trnH sequences. J Syst & Evol, 2008, 46 (2): 213~219
8 Tang SQ, Bin XY, Zhong Y. Assessment of genetic diversity in cultivars and wild accessions of Luohanguo (Siraitia grosvenorii [Swingle] A. M. Lu et Z. Y. Zhang), a species with edible and medicinal sweet fruits endemic to southern China, using RAPD and AFLP markers. Gen Res & Crop Evol, 2007, 54: 1053~1061
9 Li ZJ (李振基), Lin P (林鹏), Ye W (叶文), Chen LZ (陈鹭真), Qiu L (裘丽), Chen SB (陈圣宾), Liu CD (刘初钿), He JY (何建源), Dai DS (戴德升), Li L (李林). The biodiversity flow of ferns from southern to northern Wuyi Moutains. Prog Nat Sci (自然科学进展), 2006, 16 (8): 959~964
10 Wang J (王峻), Pan SL (潘胜利), Shun QS (顺庆生), Wang GY (王光远). A Weapon to Senile Dementia-Hup A and the Medicinal Plants of Huperziaceae in China. Shanghai, China: Shanghai Scientifc & Technological Literatre Publishing House (上海: 上海科技文献出版社), 2008. 25
11 Zhang JC (张君诚), Song YH (宋育红), Huang H (黄晖), Zhang HY (张杭颖), Chen JM (陈建民). Investigation on the feature of Huperziaceae community at Sanming Area in Fujian province. J Fudan Univ (复旦学报自然科学版), 2008, 47 (5): 627~632
12 Doyle JJ, Doyle JL. A rapid DNA isolation procedure for small quantities of fresh leaf material. Phytochem Bull, 1987, 19: 11~15
13 Yeh FC, Yang R, Boyle TJ. PopGene32, Microsoft Windows-based Freeware for Population Genetic Analysis (version 1.32). Alberta, Canada: Molecular Biology and Biotechnology Centre, University of Alberta, 2000
14 Peakall R, Smouse P. GENALEX 6: Genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes, 2006, 6: 288~295
15 Vekemans X. AFLP-SURV (version 1.0). Distributed by the author. Brussels, Belgium: Laboratoire de Génétique et Ecologie Végétale, Université Libre de Bruxelles, 2002
16 Zhivotovsky LA. Estimating population structure in diploids with multilocus DNA markers. Mol Ecol, 1999, 8: 907~913
17 Felsenstein J. PHYLIP (Phylogeny Inference Package, version 3.6). Distributed by the author. Seattle, USA: Department of Genome Sciences, University of Washington, 2004
18 Ye W (叶文), Li ZJ (李振基), Benitoc Tan, Chen LZ (陈鹭真), He JY (何建源), Liu CD (刘初钿). Study on the biodiversity of ferns from southern to northern Wuyi Moutains. J Xiamen Univ (厦门大学学报自然科学版), 2007, 46 (3): 431~437
19 Buso GSC, Rangel PH, Ferreira ME. Analysis of genetic variability of South American wild rice populations (Oryza glumaepatula) with isozymes and RAPD markers. Mol Ecol, 1998, 7: 107~117
20 Ennos RA. Estimating the relative rates of pollen and seed migration among plant populations. Heredity, 1994, 72: 250~259
21 Yan J (闫娟), Chu HJ (楚海家), Wang HC (王恒昌), Li JQ (李建强). Genetic structure and diversity of Medicago lupulina populations in northern and central China based on EST-SSRs markers. Biodiv Sci (生物多样性), 2008, 16 (3): 263~270
22 Govindaraju DR. Relationship between dispersal ability and levels of gene flow in plants. Oikos, 1988, 52: 31~35
23 Loveless MD, Hamrick JL. Ecological determinants of genetic structure in plant populations. Ann Rev Ecol Syst, 1984, 15: 65~95
24 Chen YY (陈媛媛), Ye QG (叶其刚), Li ZZ (李作洲), Huang HW (黄宏文). Genetic structure of Xiuning population of Isoetes sinensis, a critically endangered species in China. Biodiv Sci (生物多样性), 2004, 12 (6): 564~571
25 Chen JM (陈进明), Wang JY (王晶苑), Liu X (刘星), Zhang YW (张彦文), Wang QF (王青锋). RAPD analysis for genetic diversity of Isoetes sinensis. Biodiv Sci (生物多样性), 2004, 12 (3): 348~353
26 Chen JM (陈进明), Liu X (刘星), Wang QF (王青锋). Genetic diversity in Isoetes yunguiensis, a rare and endangered endemic fern in China. Wuhan Univ J Nat Sci (武汉大学学报理学版), 2005, 51 (6): 767~770
27 Zhou HG (周厚高), Xie YL (谢义林), Li H (黎桦), Zhou Q (周琼), Zhang XL (张西丽), Wang ZR (王中仁), Zhou SL (周世良). Analysis of genetic diversity on Pteris vittata L. populations from Guangxi limestone area. Guihaia (广西植物), 2002, 22 (1): 67~71
28 Wang KQ (王可青), Wang ZR (王中仁). The allozymic variation in diploid Asplenium sarelii Hook. (Aspleniaceae). Acta Gen Sin (遗传学报), 1998, 25 (5): 454~463
29 Pang LQ (潘丽芹), Ji H (季华), Chen LQ (陈龙清). Genetic diversity of the natural populations of Adiantum reniforme var. sinense. Biodiv Sci (生物多样性), 2005, 13 (2): 122~129
30 Nybom H. Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants. Mol Ecol, 2004, 13: 1143~1155
31 Ma DW (马丹炜), Wang SH (王胜华), Luo T (罗通), Wang WG (王文国), Zhuang GQ (庄国庆), Chen F (陈放). Comparitive study on genetic diversity of rock plant Pogonatherum paniceum using ISSR and AFLP markers. Chin J Appl Environ Biol (应用与环境生物学报), 2006, 12 (5): 605~608
32 Chen JM, Wang JY, Liu X, Gituru WR, Wang QF. RAPD analysis for genetic variation within the endangered quillwort Isoetes hypsophila (Isoetaceae). Wuhan Univ J Nat Sci, 2005, 10 (2): 455~459
33 Hsu TW, Moore SJ, Chiang TY. Low RAPD polymorphism in Archangiopteris itoi, a rare and endemic fern in Taiwan. Bot Bull Acad Sin, 2000, 41 (1): 15~18
34 Ge S (葛颂), Hong DY (洪德元). Studies of morphological and allozyme variation of the endangered Adenophora lobophylla and its widespread congener A. potaninii. Acta Gen Sin (遗传学报), 1999, 26 (4): 410~417
35 Hamrick JL, Godt MJW. Allozyme diversity in plant species. In: Brown ADH, Clegg MT, Kahler AL eds. Plant Population Genetics, Breeding and Genetic Resources. Sunderland, USA: Sinauer Assoc Inc, 1989. 43~63
36 Ge XJ, Zhang LB, Yuan YM, Hao G, Chiang TY. Strong genetic differentiation of the East-Himalayan Megacodon stylophorus (Gentianaceae) detected by inter-simple sequence repeats (ISSR). Biodiv & Conserv, 2005, 14: 849~861
37 Whittier DP. Gametophytes of four tropical, terrestrial Huperzia species (Lycopodiaceae). Am Fern J, 2006, 96: 54~61
38 Wang XL (王小理), Li WK (王可青), Zhou JY (周吉燕). Morphological study on gemmae and gemmalings of Huperzia javanica (Huperziaceae). Acta Bot Yunnanica (云南植物研究), 2007, 29 (5): 521~526
39 Bussell JD. The distribution of random amplified polymorphic DNA (RAPD) diversity amongst populations of Isotoma petraea (Lobeiaceae). Mol Ecol, 1999, 8: 775~789
40 Hogbin PM, Peakall R. Evaluation of the contribution of genetic research to the management of the endangered plant Zieria prostrate. Conserv Biol, 1999, 13: 514~522
41 Cheng HY (程宏毅), Bao YX (鲍毅新), Chen L (陈良), Hu ZY (胡知渊), Ge BM (葛宝明). Effects of habitat fragmentation on gene flow of the black muntjac (Muntiacus crinifrons). Acta Ecol Sin (生态学报), 2008, 28 (3): 1109~1118

相似文献/References:

[1]翁曼丽,谢纬武,伏健民,等.新一代分子标记技术──AFLP[J].应用与环境生物学报,1996,2(04):424.
 Weng Manli,Xie Weiwu,Fu Jianmin,et al.THE MOLECULAR MARKER TECHNIQUE FOR NEXT GENERATION──AFLP[J].Chinese Journal of Applied & Environmental Biology,1996,2(01):424.
[2]张正银,刘波洋,陈放,等.麻疯树AFLP体系的建立与优化[J].应用与环境生物学报,2009,15(02):280.[doi:10.3724/SP.J.1145.2009.00280]
 ZHANG Zhengyin,LIU Boyang,CHEN Fang & TANG Lin**.Establishment of the Optimized AFLP Analysis System for Jatropha curcas[J].Chinese Journal of Applied & Environmental Biology,2009,15(01):280.[doi:10.3724/SP.J.1145.2009.00280]
[3]王兴红,高宝嘉,南宫自艳.松毛虫遗传多样性研究中AFLP反应体系的建立[J].应用与环境生物学报,2009,15(06):875.[doi:10.3724/SP.J.1145.2009.00875]
 WANG Xinghong,GAO Baojia,NANGONG Ziyan.Establishment of AFLP Fingerprinting in Genetic Diversity Analysis of Pine Caterpillars[J].Chinese Journal of Applied & Environmental Biology,2009,15(01):875.[doi:10.3724/SP.J.1145.2009.00875]
[4]李红岩,高宝嘉,南宫自艳,等.河北省黄顶菊4个地理种群遗传结构分析[J].应用与环境生物学报,2010,16(01):67.[doi:10.3724/SP.J.1145.2010.00067]
 LI Hongyan,GAO Baojia,NANGONG Ziyan,et al.Genetic Structure of Four Populations of Flaveria bidentis in Hebei, China[J].Chinese Journal of Applied & Environmental Biology,2010,16(01):67.[doi:10.3724/SP.J.1145.2010.00067]
[5]宋育红,张君诚,张杭颖.长柄石杉不同地理居群叶绿体DNA trnL-trnF序列变异与聚类分析[J].应用与环境生物学报,2014,20(03):443.[doi:10.3724/SP.J.1145.2014.11033]
 SONG Yuhong,ZHANG Juncheng,ZHANG Hangying.Analysis of cpDNA trnL-trnF sequences of Huperzia serrata var. longipetiolata of different geographical populations[J].Chinese Journal of Applied & Environmental Biology,2014,20(01):443.[doi:10.3724/SP.J.1145.2014.11033]

备注/Memo

备注/Memo:
福建省科技厅重点科技项目(No. 2010N0027)、福建省自然科学基金(No. 2008J0301)和福建省高校服务海西重点项目(No. HX200801)资助
更新日期/Last Update: 2011-02-28