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[1]杨婧 栾云鹏 卢治宇 白健冬 章洪彬 李炫莹 马桥 曲媛媛**.Trichoderma sp. WL-Go 细胞提取物合成纳米金影响因素及其催化特性*[J].应用与环境生物学报,2019,25(06):1-8.[doi:10.19675/j.cnki.1006-687x.2019.02031]
 YANG Jing,LUAN Yunpeng,LU Zhiyu,et al.Influencing factors and c atalytic characteristics of gold nanoparticles biosynthesized by the cell-free extracts of Trichoderma sp. WL-Go *[J].Chinese Journal of Applied & Environmental Biology,2019,25(06):1-8.[doi:10.19675/j.cnki.1006-687x.2019.02031]
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Trichoderma sp. WL-Go 细胞提取物合成纳米金影响因素及其催化特性*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年06期
页码:
1-8
栏目:
研究论文
出版日期:
2019-12-30

文章信息/Info

Title:
Influencing factors and c atalytic characteristics of gold nanoparticles biosynthesized by the cell-free extracts of Trichoderma sp. WL-Go *
作者:
杨婧1 栾云鹏1 卢治宇1 白健冬1 章洪彬1 李炫莹1 马桥2 曲媛媛1**
1大连理工大学环境学院,工业生态与环境工程教育部重点实验室? 大连 116024
2大连海事大学环境科学与工程学院,环境系统生物学研究所 大连 116026
Author(s):
YANG Jing1;? LUAN Yunpeng1;? LU Zhiyu1;? BAI Jiandong1;? ZHANG Hongbin 1;? LI Xuanying1;? MA Qiao2 & QU Yuanyuan1**
1 Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China
2 BInstitute of Environmental Systems Biology, College of Environmental Science and Engineering, Dalian Maritime University, Dalian 116026, China
关键词:
纳米金;?生物合成;?Trichoderma sp.;?4-硝基苯酚;?催化活性
Keywords:
gold nanoparticle ;? biosynthesis;? Trichoderma sp.;? 4-nitrophenol;? catalytic activity
DOI:
10.19675/j.cnki.1006-687x.2019.02031
摘要:
与传统的物理和化学合成方法相比而言,生物法合成纳米金具备环境友好、反应条件温和、低毒等优势,近年来受到了广泛关注。利用真菌Trichoderma sp. WL-Go 细胞提取物合成纳米金,探究了氯金酸浓度、pH、温度等反应条件对纳米金合成的影响,最后对生物纳米金催化还原4-硝基苯酚的性能进行了考察。经研究得出,氯金酸浓度、pH以及反应温度对纳米金的合成具有重要影响,影响主要体现在合成速率以及纳米金稳定性方面。温度的提高可加速纳米金合成,通过TEM分析可知30 ℃和60 ℃条件下合成的纳米金平均粒径分别为15.0 nm和15.1 nm,均为球形和伪球形。对4-硝基苯酚的催化还原实验表明,在60 ℃条件下合成的纳米金催化速率优于30 ℃条件下合成的纳米金。本研究表明,利用菌株Trichoderma sp. WL-Go 细胞提取物可以合成尺寸均一且分散性良好的纳米金颗粒,且温度的提高可在加速纳米金合成的同时提升其催化活性,对生物合成纳米金的工业化应用有一定积极意义。(图4 表1 参23)
Abstract:
In recent years, compared with physical or chemical synthesis methods, biosynthesis of gold nanoparticles ( AuNPs) has attracted wide attention due to its environmentally friendly nature, mild reaction conditions, and low toxicity effects. In this study, cell-free extracts of Trichoderma sp. WL-Go were used to synthesize AuNPs. The effects of concentration of chloroauric acid, pH and temperature for the biosynthesis process were investigated, and the catalytic ability for the reduction of 4-nitrophenol by as-synthesized AuNPs was also discussed. The results showed that the concentration of chlorometalic acid, pH and temperature had important effects on the synthesis of gold nanoparticles, which mainly reflected in the synthesis rate and the stability of AuNPs. The increase of temperature could accelerate the synthesis of process. TEM images showed that the average particle size of AuNPs synthesized at 30 °C and 60 °C was 15.0 nm and 15.1 nm respectively , which were both spherical and pseudo-spherical. According to the catalytic experiment results of 4-nitrophenol reduction, it is showed that the catalytic rate of AuNPs synthesized at 60 °C was higher than those at 30 °C. In conclusion, this study shows that the cell-free extracts of Trichoderma sp. WL-Go could synthesize AuNPs with good dispersion and uniform size . The increase of temperature can accelerate the synthesis of AuNPs and enhance their catalytic activity meanwhile , which has a positive significance for the industrial application of biosynthesized AuNPs.

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备注/Memo

备注/Memo:
收稿日期Received: 2019-01-13 接受日期Accepted: 2019-04-24
*哈尔滨工业大学城市水资源与水环境国家重点实验室开放课题(ESK201529)和国家自然科学基金项目(31800091)资助 Supported by the Open Project of State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (ESK201529), and by the National Natural Science Foundation of China (31800091)
**通讯作者 Corresponding author (E-mail: qyy@dlut.edu.cn)
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更新日期/Last Update: 2019-04-26