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[1]周思婕 王平** 张敏 陈舒展 许雯 朱丽婷 何销勤 龚书锐.酸胁迫对马尾松幼苗生长及根际铝形态的影响*[J].应用与环境生物学报,2019,25(06):1-24.[doi:10.19675/j.cnki.1006-687x.2019.01024]
 ZHOU Sijie,WANG Ping**,ZHANG Min,et al.Effects of acid stress on growth and aluminum speciation in rhizosphere of masson pine (Pinus massoniana L.) seedlings[J].Chinese Journal of Applied & Environmental Biology,2019,25(06):1-24.[doi:10.19675/j.cnki.1006-687x.2019.01024]
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酸胁迫对马尾松幼苗生长及根际铝形态的影响*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年06期
页码:
1-24
栏目:
研究论文
出版日期:
2019-12-30

文章信息/Info

Title:
Effects of acid stress on growth and aluminum speciation in rhizosphere of masson pine (Pinus massoniana L.) seedlings
作者:
周思婕 王平** 张敏 陈舒展 许雯 朱丽婷 何销勤 龚书锐
南京林业大学生物与环境学院 南京 210037
Author(s):
ZHOU Sijie;? WANG Ping**;? ZHANG Min;? CHEN Shuzhan;? XU Wen;? ZHU Liting;? HE Xiaoqin;? GONG Shurui
College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China
关键词:
酸胁迫;?马尾松;?根际;?铝形态;?根系质膜;?叶绿素
Keywords:
acid stress;? Pinus massoniana;? rhizosphere;? aluminum speciation;? root plasma membrane;? chlorophyll
DOI:
10.19675/j.cnki.1006-687x.2019.01024
摘要:
在不同pH值(2.5、3.5、4.5和5.6)模拟酸雨处理下,研究根箱栽培马尾松(Pinus massoniana L.)幼苗生物量(根茎长、根与茎叶干重、一级侧根数)、叶绿素含量以及根系质膜相对透性的变化,在酸雨pH 3.5-5.6范围内对松树幼苗根际与非根际土壤的pH值和铝形态以及植株铝积累量进行测定,以探明酸胁迫对马尾松幼苗生长及根际铝形态的影响。结果显示:当酸雨pH从5.6降至2.5,马尾松幼苗生物量和叶绿素含量均降低,而根系质膜透性从13.2%升至36.4%,膜伤害度由轻度的4.1%提高到重度的23.2%,且重度酸胁迫下两者变化均极显着(P < 0.01),此外随酸胁迫强度的增强,马尾松根际与非根际土壤pH值分别由5.95和5.91降至4.02和3.87,而根际土壤中交换态、有机结合态铝含量则分别上升至7.27和18.1 mg/kg,且马尾松植株铝积累量增加至49.46 μg/株,其在植株根系中的积累量明显高于地上部分。上述结果表明,酸胁迫使得马尾松根系质膜受损程度增加,叶绿素含量减少,引起光合作用减弱,幼苗生长受到抑制;另外,酸雨能改变土壤中铝化合物的形态,增加活性铝离子溶出量,其中一部分转化成有机结合态铝,另一部分则通过根系吸收累积在幼苗植株内,阻碍马尾松生长发育。
Abstract:
To understand the effects of acid stress on the growth and aluminum speciation in rhizosphere of Masson Pine ( Pinus massoniana L.) seedlings, changes of seedling biomass (root length, stem length, root dry weight, stem and leaves dry weight, primary lateral roots number), chlorophyll content and relative permeability of root plasma membrane of Pinus massoniana cultivated in root-box cultivation were studied under simulated acid rain treatments with different pH values (2.5, 3.5, 4.5 and 5.6), besides, pH value and Aluminum speciation in rhizosphere and non- rhizosphere soil of Pinus massoniana seedlings and Aluminum accumulation of whole plant were determined under acid stress with 3.5 ≤ pH ≤ 5.6. The results showed that with the pH value of simulated acid rain decreasing from 5.6 to 2.5, the biomass and chlorophyll contents of Pinus massoniana seedlings decreased, while the permeability and injury degree of root plasma membrane increased from 13.2% to 36.4% and 4.1% to 23.2%, respectively, and the changes under severe acid stress were extremely different ( P < 0.01). In addition, with the increase of acid stress intensity, the pH values of rhizosphere and non- rhizosphere soil of Pinus massoniana decreased from 5.95 to 4.02 and 5.91 to 3.87, respectively, while the contents of exchangeable and organically bound Aluminum in rhizosphere soil increased to 7.27 and 18.1 mg kg -1, respectively, and the Aluminum accumulation of Pinus massoniana increased to 49.46 μg strain -1, and the accumulation of Aluminum ions in the roots of plant was obviously greater than that of the aerial parts. The above results indicate that acid stress increases the damage degree of root plasma membrane, reduces the chlorophyll contents which results in the weakening of photosynthesis, and inhibits the growth of Pinus massoniana seedlings. Moreover, the results demonstrate that acid rain can change Aluminum speciation in soil, increase dissolution of active aluminum ions, some of them are converted into organically bound aluminum, while others are accumulated in seedling plants through root absorption, hindering the growth and development of Pinus massoniana.

相似文献/References:

[1]周国逸,小仓纪雄.模拟酸雨的土壤淋洗液对马尾松华山松幼苗生长的影响[J].应用与环境生物学报,1995,1(02):138.
 Zhou Guoyi,Norio Ogura.EFFECTS OF SOIL SOLUTIONS LEACHED BY SIMULATED ACID RAINS ON THE GROWTH OF PINUS MASSONIANA AND P.ARMANDII SEEDLINGS[J].Chinese Journal of Applied & Environmental Biology,1995,1(06):138.
[2]潘开文,杨冬生,江心.用马尔柯夫模型预测马尾松低效林改造恢复过程[J].应用与环境生物学报,1996,2(01):29.
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 WANG Shuiliang,WANG Ping,WANG Chenyi,et al.Chemical Behavior of Aluminum in the Acidic Rhizosphere of Pinus massoniana Lamb—A Case Study on Its Cultivation in Acid Forest Soil in Chongqing, China[J].Chinese Journal of Applied & Environmental Biology,2010,16(06):523.[doi:10.3724/SP.J.1145.2010.00523]
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 LI Xun,CUI Ningjie,ZHANG Jian,et al.Mass loss of Pinus massoniana and Cinnamomum camphora leaf litter in forest gaps of different size*[J].Chinese Journal of Applied & Environmental Biology,2016,22(06):292.[doi:10.3724/SP.J.1145.2015.07026]
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 LI Xun,ZHANG Danju,ZHANG Yan,et al.The edge effect of a forest gap on decomposition of Pinus massoniana and Cinnamomum camphora leaf litter[J].Chinese Journal of Applied & Environmental Biology,2017,23(06):570.[doi:2017.02026]

备注/Memo

备注/Memo:
收稿日期 Received: 2019-01-14 接受日期 Accepted: 2019-05-05
*国家自然科学基金项目(30771696,31270680)资助
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更新日期/Last Update: 2019-05-06