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[1]王祎 杨文浩 毛艳玲 周碧青 聂三安 邢世和**.水稻生育期对不同施肥条件下黄泥田土壤无机氮及细菌群落的影响*[J].应用与环境生物学报,2019,25(06):1-10.[doi:10.19675/j.cnki.1006-687x.2019.01046]
 WANG Yi,YANG Wenhao,MAO Yanling,et al.Effect of the phenological stage of rice growth on soil soluble inorganic nitrogen and bacterial community in a yellow clayey under different fertilization patterns*[J].Chinese Journal of Applied & Environmental Biology,2019,25(06):1-10.[doi:10.19675/j.cnki.1006-687x.2019.01046]
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水稻生育期对不同施肥条件下黄泥田土壤无机氮及细菌群落的影响*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年06期
页码:
1-10
栏目:
研究论文
出版日期:
2019-12-30

文章信息/Info

Title:
Effect of the phenological stage of rice growth on soil soluble inorganic nitrogen and bacterial community in a yellow clayey under different fertilization patterns*
作者:
王祎1;?2 杨文浩1;?2 毛艳玲1;?2 周碧青1;?2 聂三安1;?3 邢世和1;?2**
1福建农林大学资源与环境学院 福州 350002
2土壤生态系统健康与调控福建省高校重点实验室 福州 350002
3福建农林大学生命科学学院 福州 350002
Author(s):
WANG Yi1;? YANG Wenhao1;? 2;? MAO Yanling1;? 2;? ZHOU Biqing1;? 2;? NIE Sanan1;? 3 & XING Shihe1;? 2**
1 College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2 Key Laboratory of Soil Ecosystem Health and Regulation, Fujian Agriculture and Forestry University , Fuzhou 350002, China
3 College of Life Sciences, Fujian Agriculture and Forestry University , Fuzhou 350002, China
关键词:
施肥模式;?水稻生育期;?土壤细菌;?数量与群落;?可溶性无机氮
Keywords:
fertilization pattern ;? rice growth stages;? soil bacteria;? abundance and community;? soluble inorganic nitrogen
DOI:
10.19675/j.cnki.1006-687x.2019.01046
摘要:
为揭示不同施肥条件下水稻生育期黄泥田土壤细菌群落的动态变化及对可溶性无机氮的影响,以33年不同施肥处理(不施肥CK、单施无机肥NPK、无机肥配施牛粪NPKM和无机肥配施秸秆NPKS)的黄泥田为研究对象,采用实时荧光定量PCR和高通量测序等技术分析水稻幼苗期、分蘖期、拔节期、扬花期和成熟期的黄泥田耕层土壤细菌数量、群落组成和结构。结果显示,黄泥田可溶性无机氮以铵态氮为主,含量为6.01-30.93 mg/kg,占可溶性无机氮总量的95%以上。细菌16S rRNA基因拷贝数为3.03×107-14.33×107/g干土。土壤优势细菌群落为变形菌门、绿弯菌门、放线菌门和酸杆菌门。偏最小二乘法判别分析表明,不同施肥处理细菌群落差异以拔节期最为明显;不同生育期细菌群落差异以无机有机肥配施处理最为明显。多元方差分析表明,水稻生育期可以解释细菌群落结构59.79%的变异,施肥处理可以解释细菌群落结构10.44%的变异,土壤铵态氮与细菌16S rRNA基因拷贝数呈显着正相关(P < 0.05)。本研究表明土壤细菌群落与土壤铵态氮存在一定的相关性,且水稻不同生育期引起的土壤细菌群落结构差异比不同施肥处理引起的细菌群落结构差异更为明显。(图3 表4 参27)
Abstract:
The aim of this study was to reveal the dynamics of soil bacterial community and its effect on soil soluble inorganic nitrogen in paddy soil during rice growth period under different fertilization patterns . A yellow clayey paddy soil with 33 years different fertilization treatments, i.e. control (CK) chemical fertilizer (NPK), chemical fertilizer combined with cow manure (NPKM) and chemical fertilizer combined with rice straw (NPKS) in Fujian province was used in this study. The bacterial abundance, diversity and community structure as well as soil soluble inorganic nitrogen in the paddy field at seedling, tillering, booting, flowering and ripening stage respectively, were analyzed by Real T ime quantitative PCR and High-throughput sequencing. The results showed that the ammonium concentration in yellow clayey paddy soil ranged from 6.01 to 30.93 mg/kg and was the main pattern of soluble inorganic nitrogen. The 16S rRNA gene copy number was ranged from 3.03×107 to 14.33×10 7 g -1 dry soil, thus t he abundance of soil bacteria changed greatly during the rice growth stages. The dominant bacterial communities in yellow clayey paddy soil were Proteobacteria, Chloroflexi, Actinobacteria and Acidobacteria. Partial Least Squares Discriminant Analysis (PLS-DA) showed that booting stage indicates great variables on bacteria communities compared with other growth stages of rice, while inorganic fertilizers combined with organic fertilizers (NPKS and NPKM) show ed great variables in comparison to different treatments. The PERMANOVA result indicated that the rice growth stages accounted for 59.79% variation of bacterial community structure, while the treatment explained 10.44% variation of bacterial community structure. The soil ammonium concentration was positively correlated with the abundance of total bacteria (P < 0.05). We concluded that soil bacterial community was related to the concentration of soil ammonium nitrogen in paddy soil. The differences of bacterial community structure were more likely to be influenced by rice growth stages rather than fertilization patterns.

备注/Memo

备注/Memo:
收稿日期 Received: 2019-01-26 接受日期 Accepted: 2019-05-08
?国家自然科学基金面上项目(41671490)和福建农林大学科技创新项目(KF2015074)资助
**通讯作者 Corresponding author (E-mail: fafuxsh@126.com)
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更新日期/Last Update: 2019-05-09