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[1]张晓兰 刘桂民** 徐海燕 李新星 吴小丽 纪庚好 李莉莎 吴晓东.热融滑塌对青藏高原北麓河荒漠草原土壤细菌群落的影响*[J].应用与环境生物学报,2019,25(06):1-12.[doi:10.19675/j.cnki.1006-687x.2019.01049]
 ZHANG Xiaolan,LIU Guimin **,et al.Effects of thaw slump on the bacterial community under desert steppe in the Beiluhe Region on the Qinghai-Tibet Plateau*[J].Chinese Journal of Applied & Environmental Biology,2019,25(06):1-12.[doi:10.19675/j.cnki.1006-687x.2019.01049]
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热融滑塌对青藏高原北麓河荒漠草原土壤细菌群落的影响*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年06期
页码:
1-12
栏目:
研究论文
出版日期:
2019-12-30

文章信息/Info

Title:
Effects of thaw slump on the bacterial community under desert steppe in the Beiluhe Region on the Qinghai-Tibet Plateau*
作者:
张晓兰1;?2 刘桂民1** 徐海燕1 李新星1 吴小丽1 纪庚好1 李莉莎1 吴晓东2
1兰州交通大学环境与市政工程学院 兰州 730070
2中国科学院西北生态环境资源研究院,冰冻圈国家重点实验室青藏高原冰冻圈观测研究站 兰州 730000
Author(s):
ZHANG Xiaolan1;? 2;? LIU Guimin 1**;? XU Haiyan 1;? LI Xinxing 1;? WU Xiaoli 1;? JI Genghao 1;? LI Lisha 1 & WU Xiaodong 2
1 School of Environmental and Municipal Engineering, Lanzhou Jiaotong University , Lanzhou 730070, China
2 Cryosphere Research Station on the Qinghai-Tibet Plateau, State Key Laboratory of Cryospheric Sciences, Northwest Institute of the Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, China
关键词:
热融滑塌;?青藏高原;?荒漠草原;?细菌群落;?多年冻土;?高通量测序
Keywords:
thaw slump;? Qinghai-Tibet an Plateau ;? desert steppe;? bacterial community;? permafrost;? High-Throughput Sequencing
DOI:
10.19675/j.cnki.1006-687x.2019.01049
摘要:
多年冻土退化会引起热融滑塌,进而对多年冻土区的生态系统产生影响,但热融滑塌对土壤细菌群落的影响还不清楚。研究区选择青藏高原中部的荒漠草原地区。利用Illumina测序技术,对土壤细菌的16S rRNA V3-V4 高变区进行测序,在纲水平上分析了3种微地貌(对照区、滑塌区、沉降区)下表层0-30 cm土壤细菌的α多样性、物种丰度和组成,并结合土壤理化指标研究了影响细菌群落结构的环境因子。结果显示,土壤细菌在纲水平上共有91个细菌类群,放线菌纲(29.4%,Actinobacteria)、酸杆菌纲(14.16%,Acidobacteria)、α-变形菌纲(12.69%,Alphaproteobacteria)和芽单胞菌纲(6.92%,Gemmatimonadetes)是优势菌群,放线菌纲在各采样点相对丰度最高。热融滑塌改变了土壤含水量、全碳和有机碳等理化指标。Mantel测试和RDA分析表明,土壤全碳和含水量是影响细菌群落结构的关键环境因子;相关性分析表明,土壤含水量、电导率、全氮是影响细菌群落多样性和优势菌纲相对丰度的关键环境因子。本研究表明在荒漠草原地区,热融滑塌会降低土壤碳含量和酸杆菌纲(Acidobacteria)的相对丰度,并对土壤细菌群落的结构、多样性及在冻土中的分布产生影响。(图6表2参36)
Abstract:
Permafrost degradation leads to thaw slumps, and thus further affects permafrost ecosystems. However, the effects of thaw slump on bacterial community largely remains unknow. We selected a desert steppe area in the central Qinghai-Tibetan Plateau. The Illumina sequencing technique was used to examine the soil bacterial communities. The sequence region was 16S rRNA V3-V4 hypervariable region of bacteria. At the Class level, the α diversity, relative abundances, and community composition of the soil bacterial under three micro-morphologies (control, collapsing, slumped) were examined. The soil physicochemical properties were also investigated to explore the main environmental factors affecting the bacterial community structure. At the class level, 91 classes were observed, the dominant classes were Actinobacteria (29.4%) , Acidobacteria (14.16%) , α-Proteobacteriaand (12.69%) and Gemmatimonadetes (6.92%) . The Actinobacteria are the most abundant in the soils of the three landforms. The results showed that the thaw slump dramatically changed the physicochemical properties of the soil, including soil water content, total carbon and organic carbon content. The Mantel test and RDA analysis showed that soil total carbon and water content were the driving environmental factors affecting the bacterial community struct ure; Correlation analysis showed that soil water content, conductivity, and total nitrogen were the driving factors of the bacterial diversity and the relative abundance of dominant classes. This study showed that thaw slump in desert steppe area decreased soil carbon contents and the relative abundance of Acidobacteria. The bacterial structure, diversity and distribution of soil bacteria were also greatly affected by thaw slumps.

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备注/Memo

备注/Memo:
收稿日期 Received: 2019-01-27 接受日期 Accepted: 2019-04-11
*国家自然科学基金项目(41661013,41871060,41861011)资助 Supported by the National Natural Science Foundation of China (41661013, 41871060, 41861011)
**通讯作者 Corresponding author (E-mail: liuguimin@mail.lzjtu.cn)
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更新日期/Last Update: 2019-04-12