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[1]鲁少文 魏翠翠 李泽华 何晓红 陶勇**.新型己酸菌强化对人工窖泥培养过程中的原核微生物群落结构及酸、酯含量的影响[J].应用与环境生物学报,2019,25(06):1-13.[doi:10.19675/j.cnki.1006-687x.2019.03036]
 LU Shaowen,WEI Cuicui,et al.Effects of a new caproic acid-producing bacteria on prokaryotic community structure and the content of acid and ester during the culture of artificial pit mud*[J].Chinese Journal of Applied & Environmental Biology,2019,25(06):1-13.[doi:10.19675/j.cnki.1006-687x.2019.03036]
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新型己酸菌强化对人工窖泥培养过程中的原核微生物群落结构及酸、酯含量的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年06期
页码:
1-13
栏目:
研究论文
出版日期:
2019-12-30

文章信息/Info

Title:
Effects of a new caproic acid-producing bacteria on prokaryotic community structure and the content of acid and ester during the culture of artificial pit mud*
作者:
鲁少文1;?2 魏翠翠1;?2 李泽华1;?2 何晓红1 陶勇1**
1中国科学院环境与应用微生物重点实验室&四川省环境微生物重点实验室,中国科学院成都生物研究所 成都 610041
2中国科学院大学 北京 100049
Author(s):
LU Shaowen1;? 2;? WEI Cuicui1;? 2;? LI Zehua1;? 2;? HE Xiaohong1 & TAO Yong1**
1Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences & Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu 610041, China
2University of Chinese Academy of Sciences, Beijing 100049, China
关键词:
人工窖泥;?瘤胃梭菌;?16S rDNA;?冗余分析(RDA)
Keywords:
artificial pit mud;? Clostridium sp .CPC-11;? 16S rDNA;? r edundancy analysis (RDA)
DOI:
10.19675/j.cnki.1006-687x.2019.03036
摘要:
人工窖泥对于浓香型白酒的生产酿造起着至关重要的作用。前期研究中,我们首次分离到能够利用乳酸合成己酸的新型产己酸菌,一种瘤胃梭菌(Clostridium sp. CPC-11,CGMCC No:9926),该菌属于瘤胃菌科(Ruminococcaceae)中Clostridium cluster IV的一个新种。本文通过16S rDNA高通量测序技术(MiSeq)结合有机酸与酯类含量分析研究了CPC-11在强化人工窖泥的制作过程中,对窖泥微生物群落结构和理化指标的影响。结果显示,经35天培养,窖泥微生物群落多样性显着降低,表明窖泥中杂菌不断减少,有益菌不断增加。其中,芽孢乳杆菌科(Sporolactobacillaceae)与瘤胃菌科(Ruminococcaceae)成为最主要的优势类群,丰度分别66.2%和22.8%。窖泥中己酸的含量在前21天不断升高,但在35天显着下降( P <0.05);乳酸和乙醇呈不断升高趋势,而乙酸则缓慢下降,丁酸未有显着改变。酯类分析显示,四大酯类(乙酸乙酯、乳酸乙酯、丁酸乙脂和己酸乙酯)积累量均呈不断增加趋势。冗余分析(RDA)结果显示,窖泥中己酸含量与瘤胃梭菌(Clostridium cluster IV)呈正相关,与芽孢乳杆菌呈负相关;乳酸含量与芽孢乳杆菌、乳酸杆菌呈正相关。本研究表明,通过新型己酸菌的强化,可以在35天内使己酸菌的相对丰度保持在22.8%,并显着提高己酸乙酯在窖泥中的累积量,促进窖泥快速成熟。本研究对改进人工窖泥的制作方法和提高人工窖泥的品质提供了理论依据。(图5表3参31)
Abstract:
In the previous study, we first isolated a Clostridium sp. CPC-11(CGMCC No: 9926) capable of producing caproic acid from lactic acid, which belongs to a new species of Clostridium cluster IV in the Ruminococcaceae family. In order to know the effect of the strain on the microbial community structure and acid, ester content in the process of culture of the artificial pit mud (APM), we designed this experiment. In this experiment, strain CPC-11 was added into the APM, and its prokaryotic community at different culture periods was investigated by 16S rDNA high-throughput sequencing technology ( MiSeq). Additionally, the contents of organic (e.g., lactic acid, acetic acid, caproic acid , butyrate) and ethyl esters (e.g., ethyl hexanoate, ethyl butyrate, ethyl lactate and ethyl acetate) in the APM were measured. The results showed that after 35 days of culture, microbial community diversity in the APM decreased greatly. It indicated that maybe undesired?microbial populations declined while the desired microbes increased in the APM. In particular, both Sporolactobacillaceae and Ruminococcaceae became the dominant groups, with abundance of 66.2% and 22.8%, respectively. The content of caproic acid in the APM increased continuously until 21 days, and decreased at 35 days; lactic acid and ethanol showed an increasing trend, while acetic acid decline d slowly , butyrate did not change significantly. Ester analysis showed that the four major esters such as ethyl hexanoate, ethyl butyrate, ethyl lactate and ethyl acetate increased with the increasing time. Redundancy analysis (RDA) showed that the content of caproic acid in the APM was positively correlated with Clostridium cluster IV and negatively correlated with Lactobacillus sp.The content of lactic acid in the mud was positively correlated with Sporolactobacillus and Lactobacillus sp . This study showed that Clostridium sp.CPC-11 can maintain a relatively stable abundance (27.16-22.8%) in the APM and led to the decrease of microbial diversity in the APM, and significantly increased the accumulation of esters in the mud, indicating the novel caproic acid-producing bacterium is conducive to the rapid maturity of the APM. This study provides a theoretical basis for improving the manufacturing method of artificial pit mud and its quality.

备注/Memo

备注/Memo:
收稿日期 Received: 2019-03-19 接受日期 Accepted: 2019-04-10
*国家自然科学基金(31770090)、中国科学院环境与应用重点实验室开放课题((KLCAS-2017-01)资助
**通讯作者 Corresponding author (E-mail: taoyong@cib.ac.cn)。
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更新日期/Last Update: 2019-04-12