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[1]邰勇 邓敬轩 任洪艳** 黄振兴 阮文权.一株Agrobacterium sp.异养硝化菌的分离鉴定及其氨氧化性能*[J].应用与环境生物学报,2020,26(02):1-14.[doi:10.19675/j.cnki.1006-687x.2019.04031]
　TAI Yong,DENG Jingxuan,REN Hongyan,et al.Isolation and identification of a heterotrophic nitrifying strain of Agrobacterium sp . a nd its ammoxidation performance *[J].Chinese Journal of Applied & Environmental Biology,2020,26(02):1-14.[doi:10.19675/j.cnki.1006-687x.2019.04031]
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一株Agrobacterium sp.异养硝化菌的分离鉴定及其氨氧化性能*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年02期
页码:
1-14
栏目:
研究论文
出版日期:
2020-03-20

文章信息/Info

Title:
Isolation and identification of a heterotrophic nitrifying strain of Agrobacterium sp . a nd its ammoxidation performance *
作者:
邰勇1 邓敬轩1 任洪艳1;?2;?3** 黄振兴1;?2;?3 阮文权1;?2;?3
1江南大学环境与土木工程学院 无锡 214122
2江苏省厌氧生物技术重点实验室 无锡 214122
3江苏省水处理技术与材料协同创新中心 苏州 215009
Author(s):
TAI Yong1;? DENG Jingxuan1;? REN Hongyan1;? 2;? 3**;? HUANG Zhengxin1;? 2;? 3;? RUAN Wenquan1;? 2;? 3
1 School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China
2 Jiangsu Key Laboratory of Anaerobic Biotechnology , Wuxi 214122, China
3 Jiangsu Province Water Treatment Technology and Materials Collaborative Innovation Center , Suzhou, 215009
关键词:
异养硝化菌;?农杆菌;?氨氧化;?氨单加氧酶
Keywords:
heterotrophic nitrifying bacterium;? Agrobacterium sp.;? ammonia oxidation;? ammonia monooxygenase (AMO)
DOI:
10.19675/j.cnki.1006-687x.2019.04031
摘要:
针对传统污水处理工艺中存在的工艺复杂、脱氮效率低等问题，从江苏无锡市桃花山垃圾渗滤液生化反应池活性污泥中富集、分离及筛选出一株异养硝化菌BT1。通过16S rRNA序列分析，对分离菌株进行鉴定，同时对其异养硝化特性、氨氧化功能基因及氨氧化性能影响因素进行了研究。结果表明，分离到的异养硝化菌为农杆菌属Agrobacterium sp .。该菌经过32 h培养后，NH4+-N去除率为99.77%；TN去除率为96.99%。其中，59.62%TN转换为胞内氮，37.37%TN转化为气态氮；检测不到NO3--N和NO2--N的积累。结合氨单加氧酶基因（amoA）的PCR成功扩增，进一步证明了BT1菌株具有氨氧化能力。单因子实验结果表明，在温度为30 ℃、C/N为10-15、pH为7.0-9.0，转速为120-160 r/min的条件下，菌株均能去除98.51%以上NH4+-N，体现出良好的氨氧化性能。BT1菌株能够适应较宽的氨氮负荷，在高氨氮浓度（500和1000 mg/L）下生长良好且NH4+-N去除率均超过64.69%。本研究表明BT1菌株具有高效的异养硝化性能及优异的氨氮耐受性，具有进一步处理高浓度氨氮废水的应用前景。（图9 参39）
Abstract:
The traditional wastewater treatment processes are complicat and nitrogen removal efficiency is low. In this paper, a heterotrophic nitrifying bacteria was enriched, isolated and screened from activated sludge of landfill leachate biochemical reaction tank which located in Taohuashan,Wuxi City, Jiangsu Province. The strain was identified by morphological observation and 16S rRNA sequence analysis. Meanwhile, the heterotrophic nitrification characteristics, ammonia oxidation gene function and the influencing factors of heterotrophic nitrification properties were studied. The results showed that the isolated heterotrophic nitrifying bacteria was Agrobacterium sp., and the NH4+-N removal rate could reach 99.77% after 32 h incubation, the TN removal rate was 96.99%, of which 59.62% TN was converted into cells growth as Internal nitrogen , 37.37% TN was converted into gaseous nitrogen, the accumulation of NO3--N and NO2--N was not detected. Moreover, the amplification of ammonia monooxygenase gene ( amoA) was successfully amplified. The ability of ammonia oxidation of BT1 strain was further proved. The single factor experiment results showed that the strain could remove more than 98.51% of NH4+-N under condition of 30 °C, C/N 10-15, pH 7.0-9.0 and 120-160 r/min, which could show strain’s good ammonia oxidation performance. The ammonia nitrogen tolerance of BT1 strain was better, and could grow well with 500 and 1000 mg/L ammonia nitrogen. The NH4+-N removal rate is exceeding 64.69%. The high-efficiency heterotrophic nitrification performance and excellent ammonia nitrogen tolerance could make the BT1 strain application in the actual wastewater treatment.

相似文献/References:

[1]张培玉,曲洋,杨瑞霞,等.耐盐异养硝化菌驯化方法及分离菌株鉴定[J].应用与环境生物学报,2011,17(01):121.[doi:10.3724/SP.J.1145.2011.00121]
　ZHANG Peiyu,QU Yang,YANG Ruixia,et al.Domestication of Halotolerant Heterotrophic Nitrifying Bacteria and Identification of Two Isolated Strains[J].Chinese Journal of Applied & Environmental Biology,2011,17(02):121.[doi:10.3724/SP.J.1145.2011.00121]

备注/Memo

备注/Memo:
收稿日期 Received: 2019-06-05 接受日期 Accepted: 2019-08-14
*国家水体污染控制与治理科技重大专项(2017ZX07204002)、中央高校基本科研业务费专项资金（JUSRP51512）资助
**通讯作者 Corresponding author (E-mail renhongyan@jiangnan.edu.cn)
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更新日期/Last Update: 2019-09-04