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[1]方智振 姜翠翠 周丹蓉 潘少霖 叶新福**.基于转录组的‘三月李’及其红肉突变体ARF基因家族鉴定及分析*[J].应用与环境生物学报,2019,25(06):1-12.[doi:10.19675/j.cnki.1006-687x.2019.05020]
　FANG Zhizhen,JIANG Cuicui,ZHOU Danrong,et al.Analysis of ARF gene family of ’Sanyueli’ plum ( Prunus salicina Lindl.) and its red-fleshed mutant based on transcriptome*[J].Chinese Journal of Applied & Environmental Biology,2019,25(06):1-12.[doi:10.19675/j.cnki.1006-687x.2019.05020]
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基于转录组的‘三月李’及其红肉突变体ARF基因家族鉴定及分析*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年06期
页码:
1-12
栏目:
研究论文
出版日期:
2019-12-30

文章信息/Info

Title:
Analysis of ARF gene family of ’Sanyueli’ plum ( Prunus salicina Lindl.) and its red-fleshed mutant based on transcriptome*
作者:
方智振1;?2 姜翠翠1;?2 周丹蓉1;?2 潘少霖1;?2 叶新福1;?2**
1福建省农业科学院果树研究所 福州 350013
2福建省落叶果树工程技术研究中心 福州 350013
Author(s):
FANG Zhizhen 1;?2;? JIANG Cuicui 1;?2;? ZHOU Danrong 1;?2;? PAN Shaolin 1;?2 & YE Xinfu 1;?2**
1 Fruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013 , China; Fujian Engineering and Technology
2 Research Center for Deciduous Fruit Trees, Fujian Academy of Agricultural Sciences, Fuzhou 350013 , China
关键词:
李;?转录组;?ARF家族;?生物信息学分析;?表达分析
Keywords:
Plum ;? transcriptome;? ARF family;? bioinformatics analysis;? expression analysis
DOI:
10.19675/j.cnki.1006-687x.2019.05020
摘要:
生长素反应因子（Auxin Response Factors ，ARFs）是一类在植物生长发育过程中发挥着重要作用的转录调控因子。为鉴定ARF家族基因、了解成员特征及其在李果实成熟过程中表达模式，基于‘三月李’及其红肉突变体果实成熟过程的转录组数据，采用生物信息学分析方法进行PsARF家族基因鉴定，并对其蛋白质理化特性、亚细胞定位、系统进化树、蛋白质质保守结构域以及表达模式进行分析。共鉴定得到17个PsARF家族成员。生物信息学分析结果表明PsARF家族蛋白质长度在600-1157 aa之间，分子量（Mr）在66.24 × 103 - 129.31 × 103之间，等电点介于5.44-8.31之间，均为位于细胞核的不稳定亲水蛋白质。系统进化树分析表明PsARF家族成员可分为4组。PsARF蛋白质均具有典型的B3和Auxin_resp结构域，且多数具有Aux/IAA结构域。保守基序分析表明，PsARF家族蛋白质包含15个保守基序，但非每个蛋白质均含有保守基序。10个PsARF家族基因在‘三月李’及其红肉突变体果实成熟过程中差异表达，不同基因表达模式各不相同。其中，PsARF2和PsARF16在‘三月李’和红肉突变体中的表达模式相反；PsARF10在‘三月李’果肉中表达量显着高于红肉突变体果肉中的表达量。本研究表明PsARF家族成员可能在李果实成熟过程中具有不同的功能，结果可为深入研究PsARF家族基因在李果实成熟过程中的功能奠定基础。（图5 表2 参40）
Abstract:
Auxin response factors (ARFs) are one type of essential family of transcription factors that play vital roles in plant growth and development processes. This study aims to identify ARF family genes, investigate characteristics of ARF members and their expression patterns during fruit ripening in plum. Bioinformatics methods were used to identify PsARF family genes based on transcriptome data of ’ Sanyueli’ plum and its red-fleshed mutant during fruit ripening . Additionally, protein physical and chemical properties, subcellular localization, phylogenetic tree, protein conserved domains and expression pattern of PsARF members were analyzed. In total, 17 members of PsARF family were identified . Bioinformatics analysis results showed that the length of PsARF proteins ranged from 193 to 1157aa, with the predicted molecular weight varied from 66.24 to 129.31 kD and the isoelectric point in the range of 5. 44 to 8.31. All of them were predicted to be unstable hydrophilic proteins located in the nucleus. Phylogenetic analysis revealed that PsARFs can be divided into four different groups. All PsARF proteins contain the typical B3 and Auxin_resp domain and most of them contain Aux/IAA domain. Conservative motif analysis shows that there are 15 conserved motifs in PsARF family proteins , but not every PsARF protein contains all the motifs . 10 PsARF genes were differentially expressed during fruit ripening in ’Sanyueli’ plum and its red-fleshed mutant. The expression patterns of PsARF2 and PsARF16 in ’Sanyueli’ plum were opposite to that in its red-fleshed mutant. The expression level of PsARF10 in flesh of ’Sanyueli’ plum was significantly higher than that in the flesh of the red-fleshed mutant. This study indicates that PsARF members may function differently, which laid a foundation for further study on the function of PsARF family genes during the ripening of plum fruits.

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备注/Memo

备注/Memo:
收稿日期 Received: 2019-05-13 接受日期 Accepted: 2019-06-28
*福建省自然科学基金项目（30471225），福建省省属公益类科研院所基本科研专项（2018R1013-1），国家自然科学基金项目（31801916），福建省农业科学院青年英才计划项目（YC2015-9），福建省农业科学院创新团队项目（STIT2017-1-4）资助
**通讯作者 Corresponding author (E-mail: yexinfu@126.com)
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更新日期/Last Update: 2019-07-05