|本期目录/Table of Contents|

[1]胡泳华 周媛 严重玲 刘俊敏 张维友 王勤**.铅胁迫对双孢蘑菇积累铅及抗氧化酶活性的影响*[J].应用与环境生物学报,2020,26(02):1-9.[doi:10.19675/j.cnki.1006-687x.2019.05041]
 HU Yonghua,ZHOU Yuan,YAN Chongling,et al.Effect of lead stress on lead accumulation ability and antioxidant enzyme activities of the mushroom Agaricus bisporus*[J].Chinese Journal of Applied & Environmental Biology,2020,26(02):1-9.[doi:10.19675/j.cnki.1006-687x.2019.05041]
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铅胁迫对双孢蘑菇积累铅及抗氧化酶活性的影响*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年02期
页码:
1-9
栏目:
研究论文
出版日期:
2020-03-20

文章信息/Info

Title:
Effect of lead stress on lead accumulation ability and antioxidant enzyme activities of the mushroom Agaricus bisporus*
作者:
胡泳华1;?2 周媛2 严重玲2 刘俊敏2 张维友2 王勤2**
1 闽南师范大学生物科学与技术学院 福建 漳州 363000
2 滨海湿地生态系统教育部重点实验室 厦门大学生命科学学院 福建 厦门 361102
Author(s):
HU Yonghua1;?2;? ZHOU Yuan2;? YAN Chongling2;? LIU Junmin2;? ZHANG Weiyou2 & WANG Qin2**
1 School of Biological Science and Biotechnology, Minnan Normal University, Zhangzhou, Fujian 363000, China;
2 Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361102, China
关键词:
铅胁迫;?双孢蘑菇;?积累;?抗氧化酶
Keywords:
lead stress;? Agaricus bisporus;? accumulation;? antioxidant enzymes
DOI:
10.19675/j.cnki.1006-687x.2019.05041
摘要:
铅(Pb)是环境中最普遍的重金属污染物之一,不仅直接毒害土壤生物,破坏生态结构,还可以通过食物链迁移转化,危害人体健康,因此Pb污染的治理迫在眉睫。大型真菌具有强的金属富集能力及较高的相对年生物量,因此在对Pb污染的修复中有一定优势。本文以双孢蘑菇菌丝为研究对象,采用马铃薯葡萄糖琼脂培养的方法,研究不同浓度Pb胁迫对双孢蘑菇菌丝体的生长状况、形态以及机体内抗氧化酶系统的影响。结果表明,在实验浓度下Pb对双孢蘑菇菌丝体的生长率无显着性影响,且菌丝体中Pb含量随着培养基中Pb浓度的增加而升高,达到10.40 μg/g。扫描电镜和透射电镜结果显示,Pb胁迫下菌丝体的细胞结构受到一定破坏,细胞膜透性增加。X射线能谱分析观察得到细胞中的黑色颗粒为铅,表明Pb在细胞内的富集。菌丝体中丙二醛含量随Pb浓度的增加而增加,超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)等抗氧化酶活性随着Pb浓度的增加有不同程度的反应。在酶活力达到最大时,相比对照组分别增加了56.45%、61.36%、13.08%和10.59%,说明双孢蘑菇通过调节自身抗氧化酶系统适应Pb胁迫环境,据此在Pb污染的生物修复中具有应用潜力。
Abstract:
Lead ( Pb) is one of the most common heavy metal pollutants in the environment. It not only directly poisons soil organisms and destroys the ecological structure, but also can migrate and transform through the food chain and harm human health. Therefore, the treatment of Pb pollution is extremely urgent. Due to its strong metal enrichment ability and high relative annual biomass, macrofungi have certain advantages in the remediation of heavy metal pollution. In the present study, the mycelium of Agaricus bisporus was exposed to di fferent concentrations of Pb to evaluate its growth, morphology and the activity of antioxidant enzymes. The results showed that in the experimental concentrations of Pb, the mycelium growth had no significant effect, and the accumulation of Pb was found to increase in a concentration-dependent manner reached to 10.40 μg/g. The results of scanning electron microscopy and transmission electron microscope showed that the cell structure of mycelium was damaged and the membrane permeability was increased under Pb treatment. The black particles in the cells were found to be lead by X-ray energy dispersive microanalysis (EDX) which indicated that Pb was accumulated in the cell. Malondialdehyde contents increased with increasing Pb concentration. The activities of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase ( APX) have different degrees of response with the increaseing of Pb concentration. When the enzyme activity reached its maximum, it increased by 56.45%, 61.36%, 13.08% and 10.59%, respectively, compared with the control. This study signifies that A. bisporus can adjust its own antixodant enzyme stystem to adapt to Pb stress, suggesting that A. bisporus has application potential for the remediation of Pb.

相似文献/References:

[1]刘碧英,潘远智,赵杨迪,等.藿香蓟(Ageratum conyzoides)对土壤铅胁迫的生理响应[J].应用与环境生物学报,2011,17(05):651.[doi:10.3724/SP.J.1145.2011.00651]
 LIU Biying,PAN Yuanzhi,ZHAO Yangdi,et al.Physiological Responses of Ageratum conyzoides to Lead Stress in Soil[J].Chinese Journal of Applied & Environmental Biology,2011,17(02):651.[doi:10.3724/SP.J.1145.2011.00651]
[2]郭亚萍,张国庆,陈青君,等. 双孢蘑菇堆肥过程中细菌群落结构分析[J].应用与环境生物学报,2014,20(05):832.[doi:10.3724/SP.J.1145.2014.03020]
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[5]何海燕,张丹** 徐露,高嘉宁,等.镉胁迫下双孢蘑菇发酵液中低分子量有机酸分泌量与镉含量变化*[J].应用与环境生物学报,2020,26(01):1.[doi:10.19675/j.cnki.1006-687x.2019.03042]
 HE Haiyan,ZHANG Dan**,et al.Changes of low molecular weight organic acids secretion and cadmium content in the fermentation broth of Agaricus bisporus under cadmium stress*[J].Chinese Journal of Applied & Environmental Biology,2020,26(02):1.[doi:10.19675/j.cnki.1006-687x.2019.03042]

备注/Memo

备注/Memo:
收稿日期 Received: 2019-05-26 接受日期 Accepted: 2019-07-22
*福建省教育厅中青年教师教育科研项目(JAT170364)、国家自然科学基金项目(31530008和318704832)资助
**通讯作者 Corresponding author (E-mail: qwang@xmu.edu.cn)
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更新日期/Last Update: 2019-07-30