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[1]张富美 侯 瑞**.一株槭射脉革菌MY51的分离鉴定及脱色[J].应用与环境生物学报,2020,26(02):1-11.[doi:10.19675/j.cnki.1006-687x.2019.05052]
 ZHANG Fumei & HOU Rui **.Isolation and identification of strain Phlebia acerina MY51, and its decolorization to dyes*[J].Chinese Journal of Applied & Environmental Biology,2020,26(02):1-11.[doi:10.19675/j.cnki.1006-687x.2019.05052]
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一株槭射脉革菌MY51的分离鉴定及脱色()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年02期
页码:
1-11
栏目:
研究论文
出版日期:
2020-03-20

文章信息/Info

Title:
Isolation and identification of strain Phlebia acerina MY51, and its decolorization to dyes*
作者:
张富美?侯 瑞**
贵州大学林学院贵阳550025
Author(s):
ZHANG Fumei & HOU Rui **
College of Forestry, Guizhou University, Guiyang 550025, China
关键词:
生真菌;?白腐真菌;?槭射脉革菌;?木质素降解酶;?染料脱色
Keywords:
endophytic fungi;? white rot fungi;? Phlebia acerina;? lignin-degrading enzymes;? decolorization
DOI:
10.19675/j.cnki.1006-687x.2019.05052
摘要:
通过对兔眼蓝莓叶片组织中分离得到的内生真菌MY51进行鉴定和产木质素降解酶活性监测,探讨该菌株对染料的脱色能力. 通过形态学和分子生物学分类法对菌株MY51进行鉴定;对菌株MY51所产三种木质素降解酶酶活曲线进行测定;利用菌株MY51对固体条件下 8 种染料进行脱色能力的检测,筛选出较易脱色的染料后,以脱色效果较好的染料模拟污水染料,研究菌株MY51在静态条件下对不同浓度固体染料的脱色能力.菌株MY51为白腐真菌—槭射脉革菌Phlebia acerina),该菌株可以产木质素过氧化物酶、漆酶和锰过氧化物酶等木质素降解酶木质素过氧化物酶漆酶活性在第10 d达到;菌株MY51对不同的染料均有脱色效果,对活性红和活性黑的脱色效果最显着;但染料浓度较高时会对菌株MY51的脱色产生一定抑制作用10 mg/L、50 mg/L、100 mg/L、250 mg/L和500 mg/L活性红脱色率98.2%、94.5%、87.8%、88.3%和 85.6%50 mg/L、100 mg/L、250 mg/L和500 mg/L的分别 98%、93%、83.3%、74.5%和 65.5%菌株MY51对活性染料具有较好脱色能力,在染料废水处理领域具有一定的应用前景。
Abstract:
The endophytic fungus MY51 isolated from the leaf of Vaccinium ashei Reade was identified and the activity of its lignin-degrading enzyme was monitored to explore the decolorizing ability of the strain to dyes. The strain MY51 was identified by morphological and molecular biological classification methods, and the activity curves of three lignin-degrading enzymes produced by the strain MY51 were determined. The decolorization ability of strain MY51 to eight dyes under solid conditions was tested. The dye that was the easiest to be decolorized was screened out and used to simulate sewage dyes. The results show that strain MY51 is a white rot fungus-Phlebia acerina, which produces lignin-degrading enzymes such as lignin peroxidase, laccase and manganese peroxidase. The activities of lignin peroxidase and manganese peroxidase reached their maximum values, 11.05 U/L and 1.21 U/L, respectively, on the 8th day of culturing; and the activity of laccase reached its maximum value of 18.52 U/L on the 10th day. MY51 decolorized eight different dyes, and had the most significant decolorization effect on reactive red and reactive black. However, higher dye concentration inhibited the decolorization ability of strain MY51. After 15 days of decolorization, the decolorization rates of 10 mg/L, 50 mg/L, 100 mg/L, 250 mg/L and 500 mg/L reactive red were 98.2%, 94.5%, 87.8%, 88.3% and 85.6%, respectively. The decolorization rates of reactive black with mass concentrations of 10 mg/L, 50 mg/L, 100 mg/L, 250 mg/L and 500 mg/L were 98%, 93%, 83.3%, 74.5% and 65.5% respectively. Our results indicated that MY51 has good decolorization ability for reactive dyes and has a certain application prospect in the field of dye wastewater treatment.

相似文献/References:

[1]肖鹏飞,近藤隆一郎.白腐真菌对异狄氏剂的降解特性及降解机理[J].应用与环境生物学报,2013,19(01):119.[doi:10.3724/SP.J.1145.2013.00119]
 XIAO Pengfei,Kondo Ryuichiro.Degradation Characteristics and Mechanism of Endrin by a White Rot Fungus, Phlebia acanthocystis TMIC34875[J].Chinese Journal of Applied & Environmental Biology,2013,19(02):119.[doi:10.3724/SP.J.1145.2013.00119]

备注/Memo

备注/Memo:
收稿日期 Received: 2019-05-31 接受日期 Accepted: 2019-07-11
*国家自然科学基金(31801688)、贵州省科技计划项目(黔科合支撑[2017]2567)、黔科合平台人才[2018]5781)资助
**通讯作者Corresponding author (E-mail: jiayouhourui123@163.com)
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更新日期/Last Update: 2019-07-30