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[1]廖斌 徐小萍 李珊珊 梁梓豪 李汉生 林玉玲 赖钟雄**.苯丙氨酸和茉莉酸甲酯对龙眼胚性悬浮细胞柯里拉京积累的影响*[J].应用与环境生物学报,2020,26(02):1-10.[doi:10.19675/j.cnki.1006-687x.2019.06001]
 LIAO Bin,XU Xiaoping,LI Shanshan,et al.Effects of phenylalanine and methyl jasmonate on growth and corilagin accumulation of embryogenic suspension cells in Dimocarpus longan Lour.*[J].Chinese Journal of Applied & Environmental Biology,2020,26(02):1-10.[doi:10.19675/j.cnki.1006-687x.2019.06001]
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苯丙氨酸和茉莉酸甲酯对龙眼胚性悬浮细胞柯里拉京积累的影响*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年02期
页码:
1-10
栏目:
研究论文
出版日期:
2020-03-20

文章信息/Info

Title:
Effects of phenylalanine and methyl jasmonate on growth and corilagin accumulation of embryogenic suspension cells in Dimocarpus longan Lour.*
作者:
廖斌 徐小萍 李珊珊 梁梓豪 李汉生 林玉玲 赖钟雄**
福建农林大学园艺植物生物工程研究所 福州 35002
Author(s):
LIAO Bin;? XU Xiaoping;? LI Shanshan;? LIANG Zihao;? LI Hansheng;? LIN Yuling & LAI Zhongxiong**
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
关键词:
龙眼;?胚性悬浮细胞;?苯丙氨酸;?茉莉酸甲酯;?柯里拉京
Keywords:
Dimocarpus longan Lour.;? embryogenic suspension cells;? phenylalanine;? methyl jasmonate;? corilagin
DOI:
10.19675/j.cnki.1006-687x.2019.06001
摘要:
基于龙眼胚性悬浮细胞体系的建立,研究苯丙氨酸(Phe)和茉莉酸甲酯(MeJA)不同添加浓度处理对龙眼胚性悬浮细胞生长和柯里拉京积累的影响。在龙眼细胞培养第6 d添加苯丙氨酸和茉莉酸甲酯不同浓度处理,测定龙眼细胞干重、柯里拉京含量、苯丙氨酸解氨酶(PAL)和多酚氧化酶(PPO)活性,并利用qPCR技术分析柯里拉京合成相关基因DlDFR、DlLAR和DlANR的表达模式。结果显示,适宜浓度的苯丙氨酸和茉莉酸甲酯能促进龙眼胚性悬浮细胞的生长和柯里拉京的积累,最适添加浓度分别为20 mg/L和50 μmol/L,与对照组相比,柯里拉京含量分别提高了1.9倍、2.9倍,产量分别提高了4.9倍、6.8倍;苯丙氨酸和茉莉酸甲酯能诱导PAL和PPO活性提高,并可能通过诱导柯里拉京生物合成途径上DlDFR、DlLAR、DlANR基因表达下调,进而调控龙眼胚性悬浮细胞中柯里拉京的合成与积累。本研究表明,适宜浓度的苯丙氨酸和茉莉酸甲酯可诱导PAL和PPO活性提高,有效促进龙眼胚性悬浮细胞中柯里拉京含量的积累,这为今后龙眼细胞大规模培养工业化生产柯里拉京提供科学依据和理论指导。
Abstract:
In order to explore the effects of different concentrations of phenylalanine(Phe)and methyl jasmonate(MeJA)on the growth and corilagin accumulation of embryogenic suspension cells in longan. Based on the establishment of embryonic suspension culture system, the longan cells was treated with different concentrations of phenylalanine and methyl jasmonate on the 6th day of the suspension culture, after which the dry weight of longan cells, corilagin content, phenylalanine lyase (PAL) and polyphenol oxidase (PPO) activity were determined. The expression patterns of the genes related to corilagin synthesis DlDFR, DlLAR, DlANR were analyzed by real-time PCR as well. The results showed that appropriate concentrations of phenylalanine and methyl jasmonate was able to promote the growth of embryogenic suspension cells of longan and the accumulation of corilagin, and the optimum concentrations were 20 mg/L and 50 μmol/L. Moreover, compared with the control group, the content of corilagin was increased by 1.9 times and 2.9 times, and the production was increased by 4.9 times and 6.8 times, respectively. Furthermore, higher PAL and PPO activities could be induced by phenylalanine and methyl jasmonate, the synthesis and accumulation of corilagin in longan cells could be regulated through the induction of down-regulated expression of DlDFR, DlLAR and DlANR genes in corilagin biosynthesis pathway. In conclusion, appropriate concentrations of phenylalanine and methyl jasmonate could increase PAL and PPO activity, and effectively promote the accumulation of corilagin, which provide a scientific basis and theoretical guidance for the large-scale cultivation of longan cells and industrialized production of corilagin.

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备注/Memo

备注/Memo:
收稿日期 Received: 2019-06-02 接受日期 Accepted: 2019-07-29
*国家自然科学基金资助项目(31572088)、国家级大学生创新创业训练项目(201710389019) 、福建省重大科技专项专题(2015NZ0002-1)、科技创新专项基金(CXZX2017189)资助
**通讯作者 Corresponding authors(E-mail: Laizx01@163.com)
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更新日期/Last Update: 2019-07-30