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[1]吕煜梦 张舒婷 王雪晶 张梓浩 程春振 王天池** 赖钟雄**.多花黄精几丁质诱导赤霉素应答基因(CIGR)克隆及其功能*[J].应用与环境生物学报,2020,26(03):1-12.[doi:10.19675/j.cnki.1006-687x.2019.06022]
 LYU YumengZHANG ShutingWANG XuejingZHANG ZihaoCHENG ChunzhenWANG Tianchi**LAI Zhongxiong**.Cloning and Preliminary Functional Study of Chitin-inducible gibberellin-responsive(CIGR) Gene in Polygonatum cyrtonema Hua *[J].Chinese Journal of Applied & Environmental Biology,2020,26(03):1-12.[doi:10.19675/j.cnki.1006-687x.2019.06022]
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多花黄精几丁质诱导赤霉素应答基因(CIGR)克隆及其功能*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年03期
页码:
1-12
栏目:
研究论文
出版日期:
2020-06-25

文章信息/Info

Title:
Cloning and Preliminary Functional Study of Chitin-inducible gibberellin-responsive(CIGR) Gene in Polygonatum cyrtonema Hua *
作者:
吕煜梦 张舒婷 王雪晶 张梓浩 程春振 王天池** 赖钟雄**
福建农林大学园艺植物生物工程研究所 福州 350002
Author(s):
LYU YumengZHANG ShutingWANG XuejingZHANG ZihaoCHENG ChunzhenWANG Tianchi**LAI Zhongxiong**
Institute of Horticulture of Biotechnology,Fujian Agriculture and Forestry?University,Fuzhou 350002?China
关键词:
多花黄精;?CIGR基因;?亚细胞定位;?光质;?光周期;?基因表达
Keywords:
Polygonatum cyrtonema Hua ;? CIGR(Chitin-inducible gibberellin-responsive);? subcellular localization;? light quality;? photoperiod;? gene expression
DOI:
10.19675/j.cnki.1006-687x.2019.06022
摘要:
为研究多花黄精几丁质诱导赤霉素应答基因(Chitin-inducible gibberellin-responsive ,CIGR)在不同光处理下的的作用机制,以多花黄精为材料,采用全长验证的方法获得多花黄精CIGR基因的cDNA序列、gDNA序列,并对其进行生物信息学分析,再对CIGR蛋白进行亚细胞定位观察,同时对不同光质和光周期处理下CIGR基因的表达模式进行分析。结果显示,多花黄精CIGR基因cDNA序列和gDNA全长序列一致,完整的开放阅读框(ORF)长度为1 770 bp,共编码589个氨基酸,结果表明CIGR基因无内含子结构。生物信息学分析表明:CIGR具有一个GRAS结构域,属于GRAS家族,是植物特有的转录因子,为碱性蛋白,无信号肽;Motif分析表明,CIGR蛋白在物种间比较保守,保守区主要位于中部至3’末端;氨基酸序列进化分析表明,多花黄精与石刁柏的CIGR亲缘关系最近,同源性达81%。进一步亚细胞定位显示CIGR蛋白定位于细胞核,与预测结果一致。实时荧光定量PCR(qPCR)结果显示,多花黄精CIGR基因具有器官表达特异性,在叶中表达量最高。在不同光质和光周期处理下,CIGR基因在蓝光下表达量较高,在远红光下表达量低;在光周期9 h/d的处理中出现峰值。本研究表明CIGR基因可能受蓝光及短光照周期调控从而影响多花黄精叶片的发育过程。
Abstract:
Objectives: To study the mechanism of CIGR(Chitin-inducible gibberellin-responsive) gene. First,cloning CIGR gene,then bioinformatics analysis of CIGR gene and subcellular localization of CIGR protein. Finally, analysis of expression of CIGR gene under different light quality 、photoperiod treatment and different tissues in Polygonatum cyrtonema Hua. Methods: Using full-length verification to obtain the CIGR gene’s cDNA and gDNA sequence of Polygonatum cyrtonema Hua. Results: The cDNA sequence of CIGR gene and the full length of gDNA were identical,and the complete open reading frame (ORF) was 1770 bp in length, which encoded a total of 589 amino acids. The results showed that the CIGR gene had no intron structure. Bioinformatics analysis indicated that CIGR protein has a GRAS conserved, which belongs to the GRAS family that was a plant-specific transcription factor. Besides, CIGR was a basic protein with no signal peptide; Motif analysis found that CIGR protein was relatively conserved among different species, and the conserved region was mainly located at the middle to the 3’ end; Evolution analysis of CIGR amino acid sequence showed that the CIGR proteins in Polygonatum cyrtonema Hua and the Asparagus officinalis maintained a strong genetic relationship, and the homology was 81%. Further subcellular localization revealed that the CIGR protein was localized in the nucleus, consistented with the predicted results. Real-time quantitative PCR (qPCR) results showed that the CIGR gene of Polygonatum cyrtonema Hua had organ expression specificity and the highest expression in leaves. Under different light quality and photoperiod treatments, CIGR gene expressed higher under blue light and low expression under far red light, and the expression peak appeared in the treatment of 9 h/d photoperiod. Conclusions: This study showed that CIGR gene could respond to light quality and photoperiod treatment, and it was speculated that blue light and short daylight may be more suitable for the growth of Polygonatum cyrtonema Hua.

备注/Memo

备注/Memo:
收稿日期 Received: 2019-06-18 接受日期 Accepted: 2019-09-03
*福建农林大学横向科技项目(KH1702300)、福建省高原学科建设经费(102/71201801101)和福建农林大学科技创新基金(CXZX2017189,CXZX2018076,KF2015108)资助
**通讯作者Corresponding author (E-mail:wangtchi@hotmail.com;Laizx01@163.com)
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更新日期/Last Update: 2019-09-04