|本期目录/Table of Contents|

[1]郭艳飞 黄勇霖 张苗苗 蔡禄 赵秀娟**.高浓度氯化镍胁迫下酿酒酵母组蛋白H4K5去乙酰化调控金属硫蛋白基因的表达*[J].应用与环境生物学报,2020,26(02):1-10.[doi:10.19675/j.cnki.1006-687x.2019.06031]
 GUO YF,HUANG YL,ZHANG MM,et al.Deacetylation of histone H4K5 regulates the expression of metallothionein genes under high concentration of nickel chloride toxicity in Saccharomyces cerevisiae*[J].Chinese Journal of Applied & Environmental Biology,2020,26(02):1-10.[doi:10.19675/j.cnki.1006-687x.2019.06031]
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高浓度氯化镍胁迫下酿酒酵母组蛋白H4K5去乙酰化调控金属硫蛋白基因的表达*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年02期
页码:
1-10
栏目:
研究论文
出版日期:
2020-03-20

文章信息/Info

Title:
Deacetylation of histone H4K5 regulates the expression of metallothionein genes under high concentration of nickel chloride toxicity in Saccharomyces cerevisiae*


作者:
郭艳飞 黄勇霖 张苗苗 蔡禄 赵秀娟**
内蒙古科技大学生命科学与技术学院 包头 014010
Author(s):
GUO YF;? HUANG YL;? ZHANG MM ;?CAI L& ZHAO XJ**
School of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou 014010, China
关键词:
金属硫蛋白;?组蛋白乙酰化;?表观遗传;?镍胁迫
Keywords:
metallothionein;? histone acetylation;? epigenetics;? nickel stress
DOI:
10.19675/j.cnki.1006-687x.2019.06031
摘要:
金属硫蛋白(MTs)具有重金属解毒功能。组蛋白乙酰化/去乙酰化是表观遗传因子之一,并且参与基因的表达调控。然而,组蛋白乙酰化/去乙酰化在真核生物响应氯化镍胁迫过程中对金属硫蛋白基因的调控作用还不清楚。以酿酒酵母突变株H4K5R(该菌株模拟组蛋白H4赖氨酸5去乙酰化的状态)为实验材料,采用流式细胞术检测不同浓度氯化镍处理下酵母细胞的死亡率,发现突变菌株的生长状态和细胞存活率明显优于野生型菌株BY4741;qRT-PCR检测5 mmol/L氯化镍处理后,酿酒酵母金属硫蛋白基因Cup1、Crs5、Sod1及转录因子Cup2的表达量,结果显示,高浓度氯化镍胁迫下,野生型菌株中基因的表达水平均显着降低;耐镍菌株H4K5R中,金属硫蛋白基因Cup1表达量上调7.4倍,Crs5表达量显着下调2.56倍,Sod1表达量不显着上调,转录因子Cup2表达量上调3.81倍,并且表达水平均显着高于镍处理后的BY4741。研究表明,突变菌株H4K5R具有较强的镍耐受性;镍胁迫下,突变菌株中金属硫蛋白Cup1与转录因子Cup2的表达变化趋势与BY4741相反,说明组蛋白H4K5的去乙酰化可能通过改变基因表达模式,从而在酿酒酵母响应镍胁迫时发挥重要的调控作用。
Abstract:
Metallothioneins (MTs) functions heavy metal detoxification. One of the best known epigenetics factors is histone acetylation/deacetylation, which play an important role in gene expression regulation. However, it remains unclear that the regulation of MT in eukaryotes by histone acetylation/deacetylation under nickel stress. The Saccharomyces cerevisiae mutant H4K5R (the strain mimics the state of histone H4 lysine 5 deacetylation) was used as experimental material. The death rate of cells was detected by flow cytometry under different concentrations of nickel chloride. The study found that the growth and livability of the mutant strain were superior to the wild type strain under high concentration of nickel choride stress. The change at the transcriptional level of metalothionins in the H4K5-deacetylation S.cerevisiae strain was examined under nickel pressure. The amount of expression of the metallotionin genes Cup1, Crs5, Sod1 and Cup2 transcription factor was quantified by qRT-PCR under 5 mmol/L nickel chloride. The result show that the expression level of genes in wild-type strains was significantly reduced by nickel chloride treatment; in the resistant strain H4K5R, after nickel treated, the expression of metallothionein gene Cup1 was up-regulated by 7.4-fold, the expression of Crs5 was down-regulated by 2.56-fold, the expression of Sod1 was not significantly up-regulated, the expression of transcription factor Cup2 was up-regulated by 3.81-fold. And the expression levels of these genes in H4K5R was significantly higher than wild type after nickel treatment. The following conclusions were reached in this study: the mutant H4K5R has strong Ni2+ tolerance and there was obvious different extent of suppression between the wild type strain and the mutant strain both of which treated with 5 mmol/L NiCl2; the changing trends of the metallothionein gene Cup1 and Cup2 of H4K5R were opposite to BY4741, which indicated that the deacetylation of histone H4K5 regulated the expression pattern of metallothionein genes and their transcription factor Cup2 in S.cerevisiae in responses to nickel stress.

相似文献/References:

[1]方彩云,谢福莉,付玮,等.利用细菌表面展示技术构建镉耐受性的重组根瘤菌[J].应用与环境生物学报,2011,17(01):82.[doi:10.3724/SP.J.1145.2011.00082]
 FANG Caiyun,XIE Fuli,FU Wei & LI Youguo.Construction of Recombinant Rhizobial Strains with Enhanced Tolerance to Cadmium Using Bacterial Cell Surface Display[J].Chinese Journal of Applied & Environmental Biology,2011,17(02):82.[doi:10.3724/SP.J.1145.2011.00082]
[2]洪美玲,李春瑜,王力军,等.急性铜胁迫对海陆蛙蝌蚪抗氧化效应及金属解毒能力的影响[J].应用与环境生物学报,2015,21(03):494.[doi:10.3724/SP.J.1145.2014.11012]
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备注/Memo

备注/Memo:

收稿日期 Received: 2019-06-31 接受日期 Accepted: 2019-08-09
*国家自然科学基金项目(21767020,61361014)资助
**通讯作者 Corresponding author (E-mail: nmzxj1977@163.com)
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更新日期/Last Update: 2019-09-04