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[1]石小保 王星淇 车婧如 杨莉 伍俊为 罗彬彬 王梦鸽 钟云** 程春振**.甜橙CsHAC1基因克隆及其在响应HLB侵染过程中的表达*[J].应用与环境生物学报,2020,26(02):1-13.[doi:10.19675/j.cnki.1006-687x.2019.07008]
 SHI Xiaobao,WANG Xingqi,CHE Jingru,et al.Cloning and expression analysis of the CsHAC1 gene in response to Huanglongbing ( HLB) infection in sweet orange*[J].Chinese Journal of Applied & Environmental Biology,2020,26(02):1-13.[doi:10.19675/j.cnki.1006-687x.2019.07008]
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甜橙CsHAC1基因克隆及其在响应HLB侵染过程中的表达*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年02期
页码:
1-13
栏目:
研究论文
出版日期:
2020-03-20

文章信息/Info

Title:
Cloning and expression analysis of the CsHAC1 gene in response to Huanglongbing ( HLB) infection in sweet orange*
作者:
石小保1 王星淇1 车婧如1 杨莉1 伍俊为1 罗彬彬1 王梦鸽1 钟云2** 程春振1**
1福建农林大学园艺植物生物工程研究所 福建福州 350002
2广东省农业科学院果树研究所 广东广州 510640
Author(s):
SHI Xiaobao1;? WANG Xingqi1;? CHE Jingru1;? YANG Li 1;? WU Junwei1;? LUO Binbin1;? WANG Mengge 1;? ZHONG Yun 2** & CHENG Chunzhen 1**
1Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University , Fuzhou 350002, China
2Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences , Guangzhou 510640, China
关键词:
甜橙;?组蛋白乙酰转移酶;?黄龙病;?互作蛋白;?表观遗传调控
Keywords:
Sweet orange;? Histone acetyltransferase;? Huanglongbing;? Interacting protein;? Epigenetic regulation
DOI:
10.19675/j.cnki.1006-687x.2019.07008
摘要:
为揭示甜橙组蛋白乙酰转移酶1基因(CsHAC1)在柑橘黄龙病(HLB)侵染过程中的响应机制,利用PCR和RT-PCR分别克隆了该基因gDNA和cDNA序列,并进行系列生物信息学分析。同时,还对CsHAC1互作蛋白进行了预测并研究它们感染HLB的柑橘中的表达情况。结果显示,该基因编码序列(CDS)全长为5 307 bp,预测可编码含有1 768个氨基酸、无信号肽和跨膜结构的蛋白质。亚细胞定位预测的结果显示CsHAC1主要定位在细胞核。CsHAC1含有ZnF_TAZ、PHD、HAT_KAT11,ZnF_ZZ等多个保守结构域,蛋白互作预测结果显示CsHAC1与ZC3H19L、SUMOs 和HAM1L等蛋白存在互作关系。启动子顺式作用元件预测结果显示CsHAC1启动子除含有大量光响应元件外还含有一些逆境(如低温、防御和应激、厌氧等)和激素(如:ABA、生长素、SA等)相关元件。通过分析CsHAC1及其互作蛋白编码基因在感染黄龙病的柑橘根和叶片中的表达情况发现:CsHAC1在叶片中的表达受HLB诱导,且与HAM1L的表达呈显着负相关。本研究结果表明CsHAC1可能和它的互作蛋白基因一起通过表观遗传调控参与柑橘对HLB侵染的响应。(图9 表3 参48)
Abstract:
To reveal the response mechanism of Citrus sinensis histone acetyltransferase 1 gene ( CsHAC1) during the Citrus Huanglongbing (HLB) infection process , the gDNA and cDNA sequences of the gene were cloned by PCR and RT-PCR, followed by a series of bioinformatics analysis. Protein-protein interaction (PPI) prediction was also performed. Additionally, expression and correlation analysis of CsHAC1 and genes encoding proteins that predicted to interact with CsHAC1 were performed. The coding sequence (CDS) of this gene is 5307 bp in length. It was predicted to encode protein containing 1768 amino acids with no signal peptides nor transmembrane structure. Subcellular localization prediction result show that CsHAC1 is mainly localized in the nucleus. CsHAC1 contains multiple conserved domains such as ZnF_TAZ, PHD, HAT_KAT11, and ZnF_ZZ. The PPI prediction result revealed that CsHAC1 can interact with several proteins such as ZC3H19L, SUMOs and HAM1L and so on. Besides a large number of photoresponsive elements, some stress responsive (such as low temperature, defense and stress, anaerobic etc.) and phytohormone responsive (such as ABA, auxin, SA, etc.) elements were also identified in the CsHAC1 promoter by promoter cis-acting element prediction. Moreover, by analyzing the expression of CsHAC1 and its interacting protein-coding genes in sweet orange samples infected with HLB, we found that CsHAC1 expression was induced by HLB in leaf, and its expression was significant negatively correlated with that of HAM1L. CsHAC1, together with its interacting protein genes, contribute to the citrus response to HLB infection through epigenetic regulation.

备注/Memo

备注/Memo:
收稿日期 Received: 2019-07-08 接受日期 Accepted: 2019-08-01
*福建省自然科学基金项目(2017J01615)、福建省高原学科建设经费(102/71201801101)、福建省省级大学生创新创业训练项目(201910389050;201910389149)、广东省科技计划项目(2019B030316005)资助
**通讯作者 Corresponding author (E-mail: zhongyun99cn@163.com; ld0532cheng@126.com)
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更新日期/Last Update: 2019-09-04