|本期目录/Table of Contents|

[1]周应敏 詹明晔 张姝 王磊 黄炎 刘晓强 王一 张志忠**.亚成体大熊猫肠道微生物结构的季节差异及其与肠道纤维素酶活性的相关性*[J].应用与环境生物学报,2020,26(03):1-12.[doi:10.19675/j.cnki.1006-687x.2019.07027]
 ZHOU Yingmin,ZHAN Mingye,ZHANG Shu,et al.Intestinal microbial structure difference of sub-adult giant pandas in different seasons and its correlation with cellulase activity[J].Chinese Journal of Applied & Environmental Biology,2020,26(03):1-12.[doi:10.19675/j.cnki.1006-687x.2019.07027]
点击复制

亚成体大熊猫肠道微生物结构的季节差异及其与肠道纤维素酶活性的相关性*()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年03期
页码:
1-12
栏目:
研究论文
出版日期:
2020-06-25

文章信息/Info

Title:
Intestinal microbial structure difference of sub-adult giant pandas in different seasons and its correlation with cellulase activity
作者:
周应敏1 詹明晔2 张姝3 王磊2 黄炎1 刘晓强1 王一4 张志忠1**
1中国大熊猫保护研究中心大熊猫国家公园珍稀动物保护生物学国家林业和草原局重点实验室 都江堰 611830
2同济大学环境科学与工程学院上海市污染控制与生态安全研究院 上海 200092
3上海动物园 上海 200335
4沈阳动物园管理有限公司 沈阳 110000
Author(s):
ZHOU Yingmin1;? ZHAN Mingye2;? ZHANG Shu3;? WANG Lei2;? Huang Yan1;? Liu Xiaoqiang1;? Wang Yi4& ZHANG Zhizhong 1**
1Chinese Giant Panda Protection Research Center Key Laboratory of State Forestry and Grassland Administration on Conservation Biology of Rare Animals in The Giant Panda National Park, Dujiangyan 611830, China
2College of Environmental Science and Engineering in Tongji University, Institute of Pollution Control and Ecological Safety in Shanghai., Shanghai 200092, China
3 Shanghai Zoo , Shanghai 200335, China,
4 Shenyang zoo management co. LTD Liaoning , Sheng yang 110000, China
关键词:
亚成体大熊猫;?季节;?肠道微生物结构;?纤维素酶活性;?Pearson相关性分析
Keywords:
sub-adult giant panda;? season;? gut microbial structure;? cellulose activity;? pearson correlation analysis
DOI:
10.19675/j.cnki.1006-687x.2019.07027
摘要:
大熊猫以低营养高纤维的竹子作为主要食物来源,但其消化系统结构保留了肉食动物的特点,不具备消化纤维素的能力,因此肠道菌群对其消化纤维素具有重要意义。为了解不同季节亚成体大熊猫肠道微生物结构的变化及其与肠道纤维素消化能力的内在联系,通过高通量测序技术分析4只亚成体大熊猫的肠道细菌和真菌多样性的季节差异,并检测不同季节的肠道纤维素酶活性,探讨了亚成体大熊猫的肠道微生物结构的季节差异与其肠道纤维素酶活性的相关性。发现不同季节亚成体大熊猫肠道细菌、真菌结构和丰富度存在较大差异,冬季肠道微生物结构较复杂,微生物丰富度也相对较高,冬季(2月)细菌的优势度明显高于其他季节。秋季(11月)和冬季(2月),亚成体大熊猫的纤维素酶活性普遍较高,冬季纤维素酶活性约为其他季节的5倍。亚成体大熊猫的纤维素酶活与其肠道优势细菌Streptococcus丰度的季节变化具有相似的趋势,且相关性系数为0.582。此外,较复杂的肠道微生物结构,特别是真菌更有利于亚成体大熊猫消化纤维素。因此,亚成体大熊猫不同季节的肠道微生物结构演变对其纤维素消化具有重要影响。(图3 表6 参28)
Abstract:
Objectives: The giant panda takes low nutrition and high fiber bamboo as the main food , but its digestive system structure retains the characteristics of carnivores and does not have the ability to digest cellulose, so The gut microorganism of giant pandas is important for the digestion of cellulose. This study is aimed to investigate the changes of intestinal microbial structure of sub-adult giant pandas in different seasons and their inner relationship with the ability of cellulose digestion . Methods: This study used high-throughput sequencing technology to analyze the bacterial and fungal diversity of the four sub-adult giant pandas in different seasons, as well as the cellulase activity of sub-adult giant pandas in different seasons was tested. Results: Then this study investigated the gut microbial structure of sub-adult giant pandas in different seasons and its correlation with their cellulase activities. The results showed that there were significant differences in the intestinal bacteria and fungi structure and richness of sub-adult giant pandas in different seasons. The microbial structure in winter (February) was complicated and the microbial richness was relatively high. The dominant degree of bacteria in winter was significantly higher than other seasons. In autumn (November) and winter (February), the cellulase activity of sub-adult giant pandas was generally higher, and in winter was about 5 times that of other seasons. Conclusions: It was closely similar to the changes of the intestinal dominant bacteria abundance, Streptococcus, between which the correlation coefficient is 0.582. In addition, more complex intestinal microbial structures, especially fungal, are more conducive to sub-adult giant panda digestion of cellulose. In general, the evolution of intestinal microbial structure of sub-adult giant pandas in different seasons has an important impact on cellulose digestion.

备注/Memo

备注/Memo:
收稿日期 Received: 2019-07-15 接受日期 Accepted: 2019-08-30
*上海市科学技术委员会重大科技攻关课题(16dz1205903,16dz1205904)资助
**通讯作者 Corresponding author (E-mail: zhouyingmin3552@sina,com)
点击摘要页题目后的“PDF”可下载阅读全文;本文为已录用的作者修定稿,尚未经编辑全面修改。
引用本文请注明出处本刊;发表刊期和页码将以正式出版时的安排为准,但DOI确定不变。
更新日期/Last Update: 2019-09-04