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[1]赛闹汪青 曹佳鑫 庞海龙 石珍珍 冯汉青**.多壁碳纳米管对水稻幼苗的生理学效应及对1,2,4-三氯苯毒性的缓解*[J].应用与环境生物学报,2020,26(03):1-14.[doi:10.19675/j.cnki.1006-687x.2019.07037]
 SAINAO Wangqing,CAO Jiaxing,PANG Hailong,et al.Physiological responses of Oryza sativa L . seedlings to multi-walled carbon nanotubes and alleviative effects of multi-walled carbon nanotubes on toxicity of trichlorobenzene*[J].Chinese Journal of Applied & Environmental Biology,2020,26(03):1-14.[doi:10.19675/j.cnki.1006-687x.2019.07037]
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多壁碳纳米管对水稻幼苗的生理学效应及对1,2,4-三氯苯毒性的缓解*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年03期
页码:
1-14
栏目:
研究论文
出版日期:
2020-06-25

文章信息/Info

Title:
Physiological responses of Oryza sativa L . seedlings to multi-walled carbon nanotubes and alleviative effects of multi-walled carbon nanotubes on toxicity of trichlorobenzene*
作者:
赛闹汪青 曹佳鑫 庞海龙 石珍珍 冯汉青**
西北师范大学生命科学学院 兰州 730070
Author(s):
SAINAO Wangqing;? CAO Jiaxing;? PANG Hailong;? SHI Zhenzhen & FENG Hanqing**
College of Life Science, Northwest Normal University, Lanzhou 730070, China
关键词:
多壁碳纳米管;?水稻幼苗;?1;? 2;? 4-三氯苯;?氧化胁迫;?叶绿素荧光
Keywords:
Multi-walled carbon nanotubes;? Oryza sativa L . seedlings ;? 1;?2;?4-trichlorobenzene;? Oxidative stress;? Chlorophyll fluorescence
DOI:
10.19675/j.cnki.1006-687x.2019.07037
摘要:
研究多壁碳纳米管(Multi-walled carbon nanotubes,MWCNTs;1 g/L)对水稻幼苗生理学反应的影响是否具粒子尺寸依赖效应,并在受试外径范围内筛选出对水稻幼苗生理学反应无不良影响的MWCNTs,探究其在培养基质中的存在能否有效缓解1,2,4-三氯苯(TCB)对水稻幼苗的生理学胁迫作用。结果显示:将水稻幼苗暴露于不同外径MWCNTS(< 8 nm,20-30 nm,> 50 nm)的悬浮液中培养 10 d 后,与对照相比,< 8 nm 或20-30 nm MWCNTs处理抑制了水稻幼苗的生长,降低了根系可溶性糖和可溶性蛋白含量、SOD(超氧化物歧化酶)活性、POD(过氧化物酶)活性和APX(抗坏血酸过氧化物酶)活性,以及叶片PS II(光系统 II )光化学活性和叶绿素含量;而根系O2.-(超氧阴离子)积累量和CAT(过氧化氢酶)活性则上升。相较< 8 nm MWCNTs处理,20-30 nm MWCNTs 处理对幼苗上述不良影响有所减轻。然而,> 50 nm MWCNTs处理则显着优化了幼苗的生长,维持了根系活性氧(Reactive oxygen species,ROS)代谢平衡,并且提高了根系可溶性糖和可溶性蛋白含量,以及叶片 PS II 光化学活性和叶绿素含量。非 TCB (1, 2, 4-三氯苯)处理条件下,添加外源MWCNTs(> 50 nm,1g/L)显着提高了根系可溶性糖含量,叶片PS II 光化学活性和叶绿素含量;与单独TCB(40 mg/L)处理相比,TCB(40 mg/L)+ MWCNTs(> 50 nm,1g/L)复合处理显着缓解了TCB对水稻幼苗的生理学胁迫作用。上述结果表明,MWCNTs 对水稻幼苗的生理效应具有粒子尺寸依赖性,且外径> 50 nm的MWCNTs在培养基质中的存在能够显着缓解TCB 对水稻幼苗的生理学胁迫作用。
Abstract:
In this paper , whether multi-walled carbon nanotubes (MWCNTs ; 1g/L) have a particle size-dependent effect on the physiological response of Oryza sativa L seedlings were studied, and further investigated the alleviative effects of MWCNTs, which no adverse effects on physiological responses of Oryza sativa L seedlings were screened in the outer diameter range of the test, on toxicity of trichlorobenzene (TCB) . Results showed that, after MWCNTs with two different outer-diameter ( < 8 nm and 20-30 nm, 1g /L, respectively) were applied to the roots of Oryza sativa L. seedlings grown for 10 d under hydroponic condition , the seedling growth was inhibited , and the soluble protein and soluble sugar content, SOD (superoxide dismutase) activity , POD (peroxidase) activity , APX ( ascorbate peroxidase) activity of roots were decreased , compared to controls . And , such treatment also caused leaf PS II photochemistry activity and chlorophyll content were reduced . Conversely, the root O2·- ( superoxide anion) production rate and CAT (catalase) activity were increased under such treatment. However , above physiological adverse effects to Oryza sativa L. seedlings was alleviated with treatment with 20-30 nm MWCNTs, compared to <8 nm MWCNTs treatment. Importantly , MWCNTs with outer-diameter >50 nm presented in medium effectively enhanced the seedlings growth, maintained the ROS ( reactive oxygen species) metabolism balance of roots, increased the soluble protein and soluble sugar content of roots , improved PS II photochemistry activity and chlorophyll content of leaves. We further investigated the alleviative effects of MWCNTs with outer-diameter > 50 nm on the toxicity of TCB (1,2,4-trichlorobenzene) to Oryza sativa L. seedlings . Without TCB stress , the MWCNTs presented in medium increased the root soluble sugar content, leaf PS II photochemistry activity and chlorophyll content. In comparison with TCB stress , the MWCNTs presented in medium significantly alleviated the TCB-induce negative physiological effects on Oryza sativa L. seedings. These results suggest that MWCNTs at moderate outer-diameter can be used as an advantageous additive during the growth of Oryza sativa L. seedlings and can protect the Oryza sativa L. seedlings from the toxicity of TCB.

相似文献/References:

[1]谈高维,韦布春,崔永亮,等.镉钝化细菌对水稻幼苗镉吸收的影响[J].应用与环境生物学报,2019,25(03):524.[doi:10.19675/j.cnki.1006-687x.201812006]
 TAN Gaowei,WEI Buchun,CUI Yongliang,et al.Effects of cadmium passivation bacteria on the growth and cadmium adsorption of rice seedlings[J].Chinese Journal of Applied & Environmental Biology,2019,25(03):524.[doi:10.19675/j.cnki.1006-687x.201812006]
[2]谈高维 韦布春 崔永亮 陈香归 闫敏 沈甜 秦诗杰 羊鑫 江鑫 余秀梅**.镉钝化细菌对水稻幼苗镉吸收的影响*[J].应用与环境生物学报,2019,25(05):1.[doi:10.19675/j.cnki.1006-687x.2018.12006]
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备注/Memo

备注/Memo:
收稿日期 Received: 2019-07-18 接受日期 Accepted: 2019-08-30
*国家自然科学基金项目(31870246,31560070)、甘肃省高等学校科研项目(2015A-007)、甘肃省重点研发计划项目(18YF1NA051)、甘肃省高校基本科研业务费和西北师范大学青年创新团队项目
**通讯作者 Corresponding author (E-mail: fenghanq@nwnu.edu.cn)
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更新日期/Last Update: 2019-09-04